几种蜂蜜对幽门螺旋杆菌的体外抗菌作用.docVIP

几种蜂蜜对幽门螺旋杆菌的体外抗菌作用 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：黄东萍， 蒋贵发， 周文誉， 毕郁， 黄文景nbsp;nbsp;nbsp;nbsp;nbsp; 【关键词】nbsp; 蜂蜜；,,幽门螺旋杆菌；,,体外抗菌作用 　　摘要：目的研究不同蜂蜜对幽门螺旋杆菌的体外抗菌作用。方法取龙眼蜜、荔枝蜜、八叶五加蜜、桂花蜜、野菊花蜜、桉树蜜、红树林蜜等7种蜂蜜，按两倍稀释法进行药敏试验，观察最低抑菌浓度和最低杀菌浓度。结果桉树蜜抑菌作用最强（MIC 1∶8），红树林蜜抑菌作用最弱（MIC 1∶2），其它蜜抑菌作用相当（MIC 1∶4）；桉树蜜还具有一定的杀菌作用（MBC 1∶2），其他蜂蜜则无此作用。结论7种蜂蜜在体外均可抑制幽门螺旋杆菌，尤以桉树蜜最佳，为开发和利用蜂蜜辅助治疗疾病提供了一定依据。 　　关键词：蜂蜜；nbsp; 幽门螺旋杆菌；nbsp; 体外抗菌作用 　　The Antibacterial Effects in Vitro of Different Kind of Honey on Helicobacter pylori 　　Abstract：ObjectiveTo study the antibacterial effects in vitro of different kinds of honey on Helicobacter pylori (Hp).MethodsSeven kinds of honey including Longan honey, Lichee honey, Acanthopanax gracilistylus honey, Sweet osmanthus honey, Wild chrysanthemum honey, Eucalyptus honey, Mangrove honey were tested. The method of doubling dilution in tubes was used in susceptibility test, the minimal inhibitory concentration and minimum bactericidal concentration were determined. ResultsEucalyptus honey had the strongest inhibitory effects on Hp which MIC was 1∶8; Mangrove Honey had the weakest effects which MIC was 1∶2; the inhibition effects of the other kinds of honey were the same which MIC was 1∶4. Eucalyptus honey also had the bactericidal effect which MBC was 1:2, the other kind didnt have such effects. ConclusionSeven kinds of honey have the inhibitory effect on Hp in vitro, and Eucalyptus honey is the best. This test provides a reference for exploiting and making use of honey to cure diseases. 　　Key words：Honey;nbsp;nbsp; Helicobacter pylori;nbsp;nbsp; Antibacterial test in vitro nbsp;nbsp;nbsp; 　　国内外的研究证明，幽门螺旋杆菌（Hp）与慢性胃炎、消化性溃疡、MALT淋巴瘤、胃癌有密切关系［1］，所以不少学者对多种西药、中药抗 Hp的效果做过研究，以求获得具有疗效的药物。蜂蜜是人类传统而古老的天然食品和医疗药品，明代医学家李时珍在《本草纲目》中论述道：“蜂蜜，其入药之功有五：清热也，补中也，解毒也，润燥也，止痛也。”在现代医学中，国内外的学者也证实蜂蜜对多种细菌有抑制及杀灭作用［2］，并且在治疗皮肤溃疡、烧创伤等方面具有显著效果［3，4］，但其对 Hp的抗菌作用尚未见报道。本文旨在研究不同蜂蜜对 Hp的抗菌作用。 　　1nbsp; 仪器与材料 　　WP