凋亡相关基因pnas2的表达与白血病关系.docVIP

凋亡相关基因pnas2的表达与白血病关系 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：黄洪晖，朱坚轶，钟济华，王海嵘，钟华，韩洁英，陈芳源 【摘要】nbsp; 本研究旨在探讨凋亡相关基因pnas2与白血病的关系。应用RTPCR技术检测硫化砷作用前后NB4、K562、U937细胞pnas2基因的表达及急性白血病病人治疗前后pnas2基因表达的变化。结果显示： NB4细胞在硫化砷作用后pnas2表达有明显下调，并呈时间依赖性；而K562及U937细胞在硫化砷作用后pnas2基因的表达无明显变化。急性白血病病人治疗前pnas2基因表达阳性率为100%，显著高于正常对照组；经非硫化砷诱导化疗后，完全缓解病人pnas2基因的表达全部转为阴性；而未达到缓解的病例pnas2基因的表达仍为阳性，较化疗前无明显变化。结论：pnas2基因可能与硫化砷诱导APL细胞凋亡密切相关，同时其表达有可能作为急性白血病分子生物学缓解的指标之一。 【关键词】nbsp; 白血病 nbsp;nbsp;nbsp;nbsp; Correlation between Expression of ApoptosisRelated Gene pnas2 and Leukemia nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; Abstractnbsp;nbsp;nbsp; The study was purposed to explore the correlation between apoptosisrelated gene pnas2 and leukemia. The RTPCR was performed to detect the expression levels of pnas2 gene in NB4, K562, U937 cells before and after treatment with AS4S4, and to analysis the expression change of pnas2 gene in bone marrow cells from patients with acute leukemia before and after chemotherapy. The results showed that the expression of pnas2 gene in arsenic sulfide treated NB4 cellsnbsp; was down regulated innbsp; timedependentnbsp; manner, butnbsp; the same outcomenbsp; in K562 and U937 cells after being treated with AS4S4nbsp; wasnbsp; not found. The positive expression rate of pnas2 in cells from untreated patients with acute leukemia was 100%, and was significantly higher thannbsp; that in normal control group. After chemotherapy, the expression was negative in complete remission patients, whereas in noremission patients there were no significant differences of expression of pnas2 before and after treatment. It is concluded that the pnas2 gene may be closely related with apotosis of arsenic sulfide treated APL cells, and may consider as anbsp;nbsp; molecular biological remission marker in acute leukemia.nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; nbsp;nbsp;nbsp; Key wordsnbsp;nbsp;nbsp; pnas2 gene；nbsp; Arsenic Sulfide； leukemia