冷冻干燥保存血小板的实验研究.docVIP

冷冻干燥保存血小板的实验研究 nbsp;nbsp; 作者：刘景汉，周俊，王冬梅，欧阳锡林，邢颜超，卢发强 【摘要】nbsp; 本研究探讨冷冻干燥保存血小板的方法，以获得可长期冻干保存的血小板制品，使之能在常温条件下保存、占用空间小、重量轻、便于长距离运输，能够满足突发事件和战伤救治的需要。在冷冻干燥保存过程中添加血小板可逆性激活抑制剂、DMSO和海藻糖等低温保护剂，进行预处理、冷冻、一级干燥、二级干燥，再水化，并同时测定血小板回收率，凝血酶聚集反应，促凝血功能，CD62p表达率和PAC1表达率等。结果表明：血小板回收率为56.29％，其对凝血酶的聚集反应与对照组无明显差异，对ADP和丙基没食子酸诱导的聚集反应较对照组分别降低49.34％和26.25％，促凝血功能与对照组比较也无统计学差异；冻干血小板CD62p表达率为42.36％，PAC1表达率为2.12％，凝血酶激活后CD62p再表达率为50.88％，PAC1再表达率为54.55％。结论：添加血小板可逆性激活抑制剂，海藻糖和DMSO后的冻干血小板，其聚集活性和促凝血功能与新鲜血小板无明显差异，血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达，增强了冻干血小板的生存能力，因而延长了其生存时间，因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。 【关键词】nbsp; 可逆性激活抑制剂 nbsp;nbsp;nbsp;nbsp; Experimental Study on Lyophilization of Platelets nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; Abstractnbsp;nbsp;nbsp; The aim of this study was to search a procedure of platelet lyophilization and find a way of longterm sto rage of human platelets at normal temperature with smallernbsp; size and lighter weight, to be convenient to transport at long distance thus to meet the demands in accidents and war time. Human platelets were pretreatednbsp; by freezing,nbsp; the first andnbsp; the second desiccation, andnbsp; were added with reversible activationinhibitors of platelets, DMSO and trehalose, then were rehydrated.nbsp; At the same time,nbsp; the recovery rate of platelets, platelet maximal aggregation induced by thrombin, coagulation of platelets, CD62p expression and PAC1 expression were assayed.nbsp;nbsp; The results indicated that the recovery rate of the plalelets was 56.29%. Thenbsp; platelet maximal aggregation induced by thrombin had no significant difference between lyophilized platelets andnbsp; the fresh plateletrich plasma (FPRP), but the aggregation of plateletsnbsp;nbsp; induced by ADP or propyl gallate was decreased by 49.34% and 26.25%. Coagulation of the lyophilized plateletsnbsp; wasnbsp; not significantly different from FPRP. CD62p expression of the lyophilized plateletsnbsp; (42.36％)nbsp; was higher thannbsp; that innbsp; FPRP