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Is Yeast Alive Teacher Prep Notes
Equipment and Supplies:*
Baker’s yeast (preferably super active or fast rising) (see Teacher Preparations 1, below)
Sugar (see Teacher Preparations 1, below)
Plastic zip-lock baggies (2 per group)
Small water balloons (4 per group)
Test tubes, between 15-25 mL (4 per group)
Test tube racks (1 per group)
Container for water that will hold at least 100 mL (1 per group)
Gloves (optional, ~2 per group)
Sharpies (1 per group)
Petri dishes (1 per group)
Yeast growth medium to fill Petri dishes
Microscope(s), slides and coverslips (2-4 per group)
Purchase from :
You can purchase a variety of plastic test tubes at for less than 10 cents each (sometimes much less). You can also purchase test tube racks for $4.35.
Purchase from Carolina Biological:
Supplies for Sterile Agar Plates—see below
63-2962 22 × 22 mm Coverslips $3.10 Box of 100
63-2950 Microscope slides $6.25 Box of 72
*If you do not have access to reasonable quality compound microscopes (yeast cells are 5-10 μm in diameter), this lab activity can be done just as well by simply omitting step 6 in the test for growth. If you have only very limited budget, supplies and equipment, you can do the first half of the lab activity (the test for metabolism) using yeast, sugar, small water balloons, and the plastic tubes used to hold single cut flowers or very small bottles which have narrow necks that will fit into the ends of the water balloons. Take care to keep the volume of whatever container you chose small enough so it and the balloons fill up with carbon dioxide within 25 minutes using a reasonable amount of yeast.
Teacher Preparations :
You will need to experiment with your particular size of test tube to determine how much yeast you need for four test tubes. We have found that approximately 1 g of yeast and 1.5-2 g of sugar per 25 mL test tube provide good results. 1 sugar packet is 4.3 g of sugar.
At least one day before class, prepare one Petri dish of yeas
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