毕业论文---复方白芍保肝颗粒生产工艺研究.docVIP

摘要 化学性肝损伤,是由化学性肝毒性物质所造成的肝损伤。大自然和人类工业生产过程中均存在一些对肝脏有毒性的物质，可引起不同程度的肝细胞坏死、脂肪变形、肝硬化和肝癌。对治疗化学性肝损伤的药物研究很多,但多为单方药物。 本文以白芍为主要原材料，以葛根、甘草、五味子、丹参为辅料，在传统中医药配方的基础上严格按照保健食品开发的相关要求及法律法规加以合理调整，进而研发制作对抗化学性肝损伤的保健食品，复方白芍保肝颗粒。文章主要通过正交实验，对提取剂浓度、提取时间、提取温度、提取剂用量、提取次数等因素进行比较，并采用紫外分光光度法，以芍药苷，五味子醇甲，葛根素的回收率为主要参考指标筛选最佳提取工艺。实验得出的最佳醇提工艺：提取剂为75%乙醇，提取三次，提取时间分别为1h，料液比分别为1:4。实验得水提最佳工艺为：提取三次，每次料液比1:6，每次时间分别为1.5h ，醇沉工艺以70%乙醇效果最佳。最后经最佳提取工艺测得，五味子醇甲收率为，1.62%；葛根素收率为，3.69%；芍药苷收率为，2.52%。回收率均在70%以上。 关键词：保肝；白芍；五味子；正交试验 Abstract Chemical liver injury, is composed of chemical liver toxic liver injury caused by. Nature and human industrial production process there are some on hepatic toxic substances, can cause varying degrees of necrosis of liver cells, fat deformation, liver cirrhosis and hepatocellular carcinoma. On the treatment of chemical liver injury of many, but for the single drug. The white peony root as main raw materials, using the root of kudzu vine, licorice, Schisandra, Salvia miltiorrhiza as auxiliary material, in the traditional Chinese medicine formula on the basis of the development of health food in strict accordance with the relevant requirements and laws and regulations to adjust, and R D production against chemical liver injury of health food products, compound white peony liver particles. This article mainly through the orthogonal experiment, the extracting agent concentration, extraction time, extraction temperature, extraction agent, extraction times and other factors were compared, and the ultraviolet spectrophotometry, the paeoniflorin, schisandrin, Hone So recovery rate as the main reference index for screening the best extraction process. Experiment results show that the optimal alcohol extraction process of extracting agent:75% ethanol, extracting for three times, extraction time were 1H, solid-liquid ratio are 1:4. Experiment of water extraction process for extracting for three times, each time: liquid ratio1:6, each time are respectively 1.5h, alcohol precipitation process wi