黄芪提取液对小鼠单核.docxVIP

黄芪提取液对小鼠单核-巨噬细胞碳廓清能力的影响摘要：目的 探讨黄芪提取液对小鼠单核-巨噬细胞碳廓清能力的影响。方法 预饲2d少量鼠粮，每天换垫料，对小鼠进行称重，分为5组（第1d），每组4只，每4只/1笼。用生理盐水和黄芪提取液分别给正常组，免疫组和高、中、低实验组小鼠连续灌胃15d，并在奇数天对免疫组、高、中、低浓度黄芪提取液三组小鼠以0.2g/100ml稀释量的CY(环磷酰胺)进行腹注。3d后取20只小鼠后进行碳廓清实验。结果 正常组与免疫组、低浓度组相比较，都有显著性。免疫组与高、中浓度相比，有显著性。结论 黄芪提取液具有增强小鼠的单核一巨噬细胞碳廓清能力的作用，提高实验小鼠免疫功能。关键字：黄芪提取液；碳廓清实验；小鼠Effects of extract of Astragalus on the ability of carbon clearance of the mononuclear macrophage in mice Abstract: Aim In order to investigate the effect of Astragalus membranaceus on the ability of carbon clearance in mice monocytes - macrophage. Methods With a small amount of rats fed on diets mice for 2 days, every day for padding, weighing the weight of mice, the mice were divided into 5 groups (first days), 4 rats in each group, each of 4/1 cage. Lavaging the normal group, immune group and high, medium, low mice 15 days with the saline and the extract of Astragalus membranaceus , and in the immune group, high, low, in three groups of mice abdominal injection of 0.2g/100ml dilution of CY (cyclophosphamide). Taking carbon clearance test for 3 days in 20 mice.Results There was significant difference between the normal group and immune group and low concentration.With the middle and high concentration of significant differences in immune group. Conclusion The extract of Astragalus enhanced mice monocytes carbon clearance ability, enhanced the immune function of experimental mice.Keywords: Astragalus Extract; carbon clearance test; mice;1 引言1 实验的研究背景中国药典规定黄芪(Radix Astragali)为豆科植物蒙古黄芪Astragalus membranace~ (Fisch．)Bge．var．mongholicus(Bge．)Hisao或膜荚黄芪Astragalus membranaceus(Fisch)Bge的干燥根［１］ 。该属植物全世界约有1600多种，主要分布于除大洋洲外的世界亚热带和温带地区。我国有130种左右，主产于东北，经北部、西北而至西南，可人药者有10种左右［1、2］。黄芪是一味常用中药，具有补气固表、利尿托毒、排脓、敛疮生肌之功效［3］。临床用于气虚乏力、食少便溏、中气下陷、久泻脱肛、便血崩漏、表虚自汗、气虚水肿、痈疽难溃、久溃不敛、血虚痿黄、内热消渴，慢性肾炎蛋白尿、糖尿病等。黄芪的化学成分主要有黄酮类、皂苷类和多糖等［4］，其中黄酮类化合物有黄酮、异黄酮、异黄烷和紫檀烷四大类，皂苷类化合物有黄芪皂苷及其大豆皂苷。另外尚含单糖、氨基酸、蛋白质、核黄素、叶酸、尼克酸、维生素D、驱油酸、亚麻酸、微量元素、香草酸、阿魏酸、异阿魏酸、对羟苯基丙烯酸、咖啡酸、绿原酸、棕榈酸、B．谷甾醇、胡萝卜苷、羽扇豆醇，正十六醇等成分［5、6、7］