StepsforNMRExperimentssmallmolecule.pptVIP

Steps for NMR Experimentssmall molecule 吳英彥 Casper Wu OUTLINE 1. 樣品配製 2. 儀器選擇 3. NMR探頭選擇 4. 勻場 5. 1D 1H NMR 6. 1D 13C NMR 7. 2D homonuclear NMR 8. 2D heteronuclear NMR 1.1 樣品配製 取用適當的樣品量(10 mg) 選擇合適之D-solvent 取用合適的NMR sample tube 配製適當的量(5mm) ?一般樣品管: 體積 ? 0.5 mL, 3 cm ?SHIGEMI樣品管: 體積 ? 0.3 mL 1.2 樣品配製 SHIGEMI樣品管 2.1 儀器選擇 2.2 儀器選擇(靈敏度) For 1H: ? = 26753 (s-1Gauss-1) = 2.6753 (s-1Tesla-1) (1Tesla = 104 Gauss) when Bo = 4.69T ? ?E = h? = (?hBo)/2? ? ? = ?Bo/2? = 2?108 (s-1) = 200 MHz 2.3 儀器選擇(靈敏度) Boltzmann equation: N?/N? = exp(-?E/kT) = exp[(?hBo)/(2?kT)] when Bo = 4.69T (2