【2017年整理】脑益康对AD大鼠学习记忆及海马NGF TrkA mRNA表达的影响.docVIP

脑益康对AD大鼠学习记忆及海马NGFTrkA mRNA表达的影响《中国老年学杂志》 2009年1月29卷2期【摘要】? 　　目的 探讨中药复方脑益康对阿尔茨海默病（AD）大鼠学习、记忆能力及海马NGFTrkA mRNA表达的影响。方法 采用脑立体定位仪自双侧Meynert核（nucleus basalis of Meynert, NBM）注射鹅膏蕈氨酸（Ibotenic acid, IBO）建立AD大鼠模型，分别给予不同剂量脑益康对AD大鼠进行治疗。采用Y迷宫刺激器分别测试大鼠的学习、记忆能力，尼氏染色法显示基底前脑尼氏小体的变化，逆转录聚合酶链式反应（RTPCR）检测海马NGFTrkA mRNA的表达。结果 脑益康能提高IBO损伤双侧NBM、建立AD大鼠模型的学习记忆能力、改善AD大鼠基底前脑病理改变、上调海马NGFTrkA mRNA表达。结论 脑益康能提高AD大鼠的学习、记忆能力，其机制与增加NGFTrkA mRNA的表达、保护胆碱能神经元有关。 【关键词】? 脑益康；阿尔茨海默病；学习；记忆；NGFTrkA 　　Effects of Naoyikang on both learning and memory ability and expression of NGFTrkA mRNA in rats with Alzheimer′s disease 　　GENG JinSong, ZHOU AiLing, MAO JiaHui, et al.?　　Medical College of Nantong University, Nantong 226001, Jiangsu, China ??? 【Abstract】Objective? To observe the effects of Naoyikang (NYK) on learning and memory ability as well as NGFTrkA mRNA expression in hippocampus of Alzheimer′s disease (AD) rats. Methods? Bilateral infusions of ibotenic acid (IBO) into nucleus basalis of Meynert (NBM) using stereotaxic apparatus were adopted to establish the rat model of AD. NYK at different dosages were administrated to AD rats. The ability of learning and memory of AD rats were tested by Ymaze. Nissl staining was applied in observing the pathological change of nissl body in basal forebrain. Expression of nerve growth factortyrosine kinase receptor A (NGFTrkA) mRNA in hippocampus was detected by reverse transcriptionpolymerase chain reaction (RTPCR). Results? NYK improved the ability of rats injected bilaterally with IBO. NYK also normalized pathological change of neurons in basal forebrain, and increased the expression of NGFTrkA mRNA in hippocampus of AD rats.Conclusions? The mechanism of NYK to enhance the ability of learning and memory in AD rats should be related to its potentiality to increase NGFTrkA mRNA expression, which may also protect the central cholinergic neurons. ??? 【Key words】Naoyikang; Alzheimer′s disease; Learning; Memory; NGFTrkA 　　胆碱能系统功能下降，尤其是基底前脑胆碱能（chol