奶牛产后代谢组学研究要点.docVIP

奶牛产后血浆1H NMR代谢组学分析 陶金忠* 郭延生 （宁夏大学农学院动物科学系，银川，750021） 摘 要：（目的）为了从代谢组角度系统揭示奶牛分娩后能量缺乏的生理机制，（方法）本研究采用1H-NMR 代谢组学技术，结合多元统计学方法探讨了奶牛分娩后不同阶段血浆小分子代谢物的变化趋势及其对能量代谢通路的影响。（结果）结果表明：奶牛分娩后第7天与分娩第1天之间血浆代谢轮廓无明显差异；分娩后第14天与第1天之间血浆代谢轮廓出现明显差异，其差异标志物为不饱和脂肪酸、胆碱和氧化三甲胺；分娩后第28天与第1天之间血浆代谢轮廓也存在明显差异，其差异标志物为不饱和脂肪酸、胆碱、氧化三甲胺、三羧酸和葡萄糖；随着分娩后时间的延长，不饱和脂肪酸、胆碱和三羧酸表达逐渐上调，而氧化三甲胺和葡萄糖表达逐渐下调。（结论）随着奶牛分娩后泌乳量的不断增加，逐渐呈现出能量负平衡状态，而机体应答性的上调了脂肪分解、脂肪酸氧化、胆碱氧化、蛋氨酸再甲基化和甲基代谢途径以缓解奶牛分娩后的能量负平衡状态。研究结果可为进一步阐明奶牛分娩后能量负平衡的生理机制提供科学依据。 关键词：奶牛；围产期；能量负平衡；代谢组学；1H核磁共振； 中图分类号: 文献标识码:A 文章编号: Study on the Plasma Metabolism Using 1H NMR in Postpartum Cows Tao Jin-zhong, GUO Yan-sheng （College of Agriculture, Ningxia University, Yinchuan, Ningxia Province, 730020, China） Abstract: (Objective) In postpartum, cows often suffer from negative energy balance，resulting in physical dysfunction. The aim of this study was to define the physiological mechanism of cows exercising metabolic energy shortage after calving. (Method) 1H-NMR technology was used for measurement of metabolites. Results were analysized in multivariate statistical methods to find out the trend of plasma metabolites changing after calving and the influence on energy metabolism pathway. (Results) results show that: the biomarkers profile of plasma was not significantly different in D 7 from D 1 after calving; but a significant difference were found between D 14 and D 1 after calving. The biomarkers are unsaturated fatty acid, choline and trimethylamine oxide; there were significant differences in biomarkers profile between D 28 and D 1 after calving with different markers of unsaturated fatty acid, choline, trimethylamine oxide, citric acid and glucose. After calving, unsaturated fatty acid, choline and tricarboxylic acid expression gradually increased, whereas trimethylamine oxide and glucose expression gradually decreased. (Conclusion) with the increasing lactating yield after calving, cows showed a negative energy balance. In response, th