Cloning And Tissue Expression Of Tnni3 Gene In Tianfu Goat.docVIP

Cloning And Tissue Expression Of Tnni3 Gene In Tianfu Goat 　　Abstract. Cardiac troponin I （TNNI3） is an inhibitory subunit of troponin-tropomyosin regulate complex. Based on the cattle TNNI3 mRNA sequences， a 773 bp fragment （JN848969） for TNNI3 of Tianfu goat was successfully cloned and sequenced in this study. Bioinformatics analysis showed that the full-length cDNA of TNNI3 gene contained a 639bp open reading frame （ORF） encoding a protein with 212 amino acids residues. Fluorescence quantitative PCR analyses demonstrate that the TNNI3 expression was highest in cardiac muscle and the relative quantity reducing with the rise of age. In summary， this study may provide more insight into the molecular structure， expression patterns and evolution of TNNI3 gene in animal. 　　Key words： TNNI3； clone； sequence analysis； tissue expression； Tianfu goat 　　Introduction 　　With the development of breeding and nutrition techniquein animals， the growth rate of livestock and poultry has been improved greatly[1]. But， the problem is that meat quality of the animals is decreasing[2]. To make certain meat quality of animals， close attention should be paid in modern livestock breeding. The diameter and type of muscle fiber will directly influence on meat tenderness， in which the types of myofiber can be classified according to their contractile nature [3]. The gene involved in myoblast differentiation and muscle contraction are considered usually as potential candidate gene for meat quality[4]. 　　Troponins are proteins involved in excitation-contraction couping[5]. Troponin I is a regulatory thin-filament protein expressed in striated muscle. It participates in the control of muscle activation by Ca2+ along with the other subunits of troponin and tropomyosin[6]. TNNI3 is restricted in its expression to atrial and ventricular cardiac muscle[7]. 　　As the TNNI3 isoform is expressed in a fiber-type-specific manner， they have been used as a model gene to study mechanisms of cardia