Research of the Isolation of E. coli in Broilers and Drug Susceptibility Testing.docVIP

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2017-01-23 发布于北京
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Research of the Isolation of E. coli in Broilers and Drug Susceptibility Testing.doc

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Research of the Isolation of E. coli in Broilers and Drug Susceptibility Testing 　　Abstract. E. coli disease in broilers epidemiological survey conducted this test for higher mortality chicken clinical examination， inspection and disease pathogens necropsy to determine the chickens infected with E. coli. The results of susceptibility testing， said Zhuang chloramphenicol， neomycin， and the ofloxacin is highly sensitive drugs. 　　Keywords： Broiler Susceptibility testing Isolation and identification of pathogenic E. coli 　　1. Introduction 　　It is understood that the chicken E. coli disease is a general term caused by a variety of pathogenic E. coli illness. Broiler E. coli disease has become one of the major infectious diseases harm aquaculture. Broiler easy season in large temperature has difference suffering from the disease. In the winter， the usual lack of good breeding management is more likely to cause disease， and for this reason is often overlooked， thus causing unnecessary economic losses. 　　E. coli is a Gram-negative bacillus spore-free， the size of 0.4-0.7μm × 2-3μm， Rounded at both ends， scattered or in pairs [2]， flagellated， and some strains can form capsular easy proliferation in normal medium， strong adaptability. The bacteria sensitive to general disinfectant， but their mucus and feces will reduce the effect of these disinfectants. The bacteria to antibiotics and other drugs can easily develop resistance. In the animal building， E. coli in water， faeces and dust can survive for several weeks or months. 　　2. The related materials 　　2.1 Diseased 　　Material from the onset of the disease in chickens has obvious symptoms of clinical diagnosis， initial diagnosis of pathological autopsy Colibacillosis chickens. （Dead time less than 24 hours in winter， summer is not over 20 hours） or dying chicken heart， liver， spleen， lymph， fallopian tubes and other organizations. 　　2.2 Medium 　　Medium containing glucose， maltose， urea， hydrogen sulfide， peptone