Arthrobotrys Oligospora N Mutation Breeding.docVIP

Arthrobotrys Oligospora N Mutation Breeding 　　Abstract. In order to apply the nematode-trapping fungi biocontrol of animal parasitic nematode， prove that mutagenesis strain of the Arthrobotrys Oligospora N spores can be passed to experimental animal digestive tract. Ion beam injected into the spores of Arthrobotrys oligospora of nematode-trapping.Experimental animals are hurled Arthrobotrys Oligospora N spores， collect the feces of experimental animals， test fungal germination， growth， reproduction and predation livestock parasitic nematode larvae in laboratory. Mutant strains of Arthrobotrys Oligospora N can be through the digestive tract in experimental animals. Such biotechnology could be explored for improving the effectiveness of the use of fungal infections to control livestock parasitic nematodes. 　　Key words： Nematode-trapping fungi；Arthrobotrys oligospora N；Through the digestive tract 　　1 Introduction 　　With the drawbacks of the a variety of the chemical drug - worming medicine been continuously awareness， the use of the predation nematode fungi carried out prevention and treatment the livestock parasitic nematode，the development of non-polluting livestock products has an extremely important significance. Test a Arthrobotrys Oligospora N through Sheep Gastrointestinal. Establish foundation for the use of nematode-trapping fungi prevention against parasitic nematodes of livestock. 　　2 MATERIALS AND METHODS 　　2.1 Test biological and equipment 　　A. oligospora N and experimental animals by the Inner Mongolia Agricultural University College of veterinary medicine to provide. 　　In accordance with the standard method，prepared 0.4 g / L corn meal agar，conidia corn grain Culture medium. 　　Injected into the ion beam device， produced by the Hefei Institute of Plasma Physics， Chinese Academy of Sciences. 　　Strongylus equines larvae，take the horse fresh stool placed on the enamel tray （20 cm x 40cm），Were cultured at 25 ℃ for 15d.Separated and collected the