米糠多糖的提取研究及米糠中水溶性维生素的测定参考资料.doc

米糠多糖的提取研究及米糠中水溶性维生素的测定 （江南大学化学与材料工程学院，江苏无锡214122） 摘要：微波浸提法研究了米糠多糖的提取，HPLC法同时测定了米糠中4种水溶性维生素。 料液比为1：8在50％微波火力条件下浸提30min，三氯乙酸去蛋白，两步去淀粉，30％醇沉，米糠多糖得率达1.41%。以体积比为88:12的0.1％磷酸水溶液-甲醇为流动相，以0.6 mL/min流速等度洗脱，在266nm波长下检测。各组分在8min能基线分离。VB1、VB6、烟酸和烟酰胺的分别为×10-6 g/g、×10-6g/g、×10-6 g/g、×10-6 g/g。Abstract: Microwave assistant extraction has been applied for polysaccharides in rice bran samples. The best technology were that the material-water ratio was 1:8, the time of microwave assistant extraction was 30 min under 50% firepower. It was proved that α-amylase could wipe off amylum effectively by two steps, and trichloroacetic acid method was the best way to wipe off albuminoid. And the ethanol concentration was 30%, the extraction rate of the water-soluble polysaccharides of rice bran was 1.41%.High performance liquid chromatography (HPLC) has been applied for the simultaneous determination of four kinds of water-soluble vitamins including vitamin B1, vitamin B6, nicotinic acid, and nicotinamide in rice bran. The separation was performed on a Hypersil C18 column (250 cm×4.6mm i.d., 5.0 μm) with a mobile phase of 0.1% H3PO4- methanol (88:12, V/V) at flow rate of 0.6 mL /min, and detection wavelength was 266 nm.. Baseline separation of four vitamins can be achieved within 8 minutes.The content of 7.7×10-6 g/g、1.3×10-6g/g、14.9×10-6 g/g、16.2×10-6 g/g for vitamin B1, nicotinic acid ,vitamin B6 and nicotinamide. Key words: rice bran; polysaccharides; microwave assistant extraction; water-soluble vitamins; high performance liquid chromatography 米糠含有稻米的64％营养物质及90％人体所需要各种营养元素[1]，包括维生素、米糠多糖、氨基酸、脂肪酸、矿物质，还有二十八碳烷醇、神经酰胺等生理功能卓越的活性物质[2]。polysaccharide，RBP）存在于稻谷的颖果皮层里，主要是指分子量不等的一类非淀粉多糖。采用不同的提取工艺会得到不同的米糠多糖组分。米糠多糖具有广泛的生理活性，在抗肿瘤，增强免疫力，降血糖，降脂方面有显著效果；水溶性维生素是一类重要的营养成分，在各种保健品[]，功能型饮料中烟酸和烟酰胺能够促进头皮血液循环，防止脱发；VB6具有美发功效；VB1具有滋养与深层清洁的作用，因此化妆品中添加微量的维生素。测定维生素的方法有紫外分光光度法[]、荧光法[]和高效液相法）[]。米糠中水溶性维生素的测定还未见报道。采用建立了米糠中的水溶性维生素的测定方法，对样品前处理方法进行了研究，对测定体系各影响因素进行了优化。方法简便快速，准确可靠，对复杂体系中水溶性维生素的测定具有重要的参