基因转录组的测定及综述.ppt

454 reads runassembly -v –s (remove primer, vector, low quallity region(q13), high coverage(20x)) Contig + Singleton Contig + Singleton Contig + Singleton Contig + Singleton Contig + Singleton Seqclean (short reads remove(100nt)) Contamination remove(microbial i90% l90%) Allele transcripts remove(i99% l-dif 10nt) Alternative splicing remove() Contig + Singleton Homopolymer remove(solexa,solid) Contig + Singleton Scaffold construct ion(cDNA meta-paired) Scaffold construct ion(cDNA meta-paired) Contig + Singleton Contig + Singleton Sequence Extension Unigene Tissues Raw reads Assembled read