骨髓基质干细胞修复软骨缺损的实验研究.docVIP

骨髓基质干细胞修复软骨缺损的实验研究 【摘要】 探寻体外诱导骨髓基质干细胞成软骨细胞的最佳细胞因子，寻求体内修复家兔软骨缺损的最为有效方案。［方法］rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养，应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子，并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物，直接种植到兔膝关节实验性关节软骨缺损处，并与对照组相比较，观察软骨修复效果。［结果］常规形态学观察，rhTGF-β1和rhIGF-I联合应用诱导的细胞在形态上类似于软骨细胞，免疫组化染色提示诱导细胞具有软骨细胞表型。凝胶复合物直接种植在体内能诱导骨髓基质干细胞向软骨细胞分化，修复缺损的软骨，缺少细胞因子的对照组软骨缺损修复效果差。［结论］rhTGF-β1和rhIGF-I联合应用可作为诱导骨髓基质干细胞向软骨细胞分化的最佳组合，骨髓基质干细胞凝胶复合物能修复软骨缺损。 【关键词】 细胞因子 骨髓基质干细胞 软骨细胞 软骨缺损 Abstract：［Objective］To retrieval a best cell factor which can induce bone marrow stromal cells (bMSCs) into chondrocyte in vitro and to explore an effective plan to repair rabbits chondrocyte defect.［Method］Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ(rhTGF-Ⅰ) were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.［Result］The cells induced by rhTGF-β1 and rhIGF-I were similar to chondrocytes in morphology, and immunohistochemical examinations showed the cells possessed phenotype of chondrocytes. RhTGF-β1 and rhIGF-I could differentiate bone marrow stromal cells into cartilage cells in vivo, and repair the articular cartilage defect. The control group could not repair the articular cartilage defect efficiently.［Conclusion］rhTGF-β1 and rhIGF-I are best group which stimulate bMSCs to differenate into cartilage cells. BMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I can repair the articular cartilage defect. Key words：cell factor; bone marrow stromal cells; chondrocyte; cartilage defect 作者简介：童培建(1961-)，男，杭州人，教授，主任医师，在读博士研究生，研究方向：关节外科，(电话)0571骨髓基质干细胞（BMSCs）中含有少量的多功能