骨髓间充质干细胞促进骨肌腱结合部愈合的初步观察.docVIP

骨髓间充质干细胞促进骨肌腱结合部愈合的初步观察 【摘要】 观察骨髓间充质干细胞对骨-肌腱结合部愈合的影响。［方法］采用骨髓穿刺、全骨髓培养法获取兔骨髓间充质干细胞。36只18周龄新西兰大白兔随机分为2组，实验组将骨髓间充质干细胞与Pluronic F-127载体材料结合后，植入兔髌骨部分切除模型，对照组只进行手术，不植入细胞。在术后6、12、18周进行X线片、大体和组织学观察评估。［结果］X线片显示术后6、12、18周实验组骨-肌腱结合部新生骨面积显著大于对照组(Plt;0.01)。大体观察可见实验组骨-腱结合部愈合较早。HE染色显示实验组术后6周有大量的纤维母细胞和类软骨细胞增生，并可见新骨形成；12周，髌腱与松质骨接触面软骨细胞移行带形成；18周，纤维软骨移行带排列更有序。Safranin 0染色显示实验组术后6周，基质染色较深，部分骨-腱结合处可见相对浓染区；12周，基质染色范围明显减少，沿类软骨细胞走行分布；18周，类软骨细胞集中的区域染色较12周有所减退。组织学检查显示实验组术后6、12、18周骨-腱结合部组织愈合明显，较对照组迅速。［结论］骨髓间充质干细胞可以促进骨-肌腱结合部细胞增生，促进其早期愈合。 【关键词】 骨髓间充质干细胞 骨-肌腱结合部 愈合 　 Abstract：［Objective］To evaluate tho effects of Bone Mesenchymal Stem Cells(BMSCs) on healing of bonetendon junction in a patellar tendon complex.［Method］The BMSCs of rabbits were harvested by bone marrow aspiration. Adherent cells were selected as BMSCs after the whole marrow was cultured.Standard partial patellectomy was conducted in 36 18weekold rabbits divided into 2 groups. The experimental group was planted into by the composite of BMSCs and Pluronic F127 while the control group was only operated without BMSCs planting into.The patellarpatellar tendon complexes were harvested at weeks 6,12,18 postoperatively for radiographic, gross and histological evaluations.［Result］Radiographic measurements showed significantly more newly formed bone at the patellar tendonpatellar healing junction in the experimental group compared with that of the controls at weeks 6,12,18(Plt;0.01 ).The gross observations showed the tissue healing in the experimental group was quicker than that of the controls. Histologically, HEstained specimen showed in the experimental group considerable fibroblast and chondrocytelike cellular proliferation, new bones formed at week 6;chondroeytes transitional zone was formed at trabecular bonepatellar tendon junction at week 12; array of fibrocartilage transitional zone was ordered at week 18. Safranin Ostained specimen showed in the experimental group matrix wa