骨髓增生异常综合征患者间充质干细胞sdf1基因的表达.docVIP

骨髓增生异常综合征患者间充质干细胞SDF1基因的表达 【摘要】 本研究探讨骨髓基质细胞衍生因子-1(SDF-1)在骨髓增生异常综合征（MDS）患者骨髓间充质干细胞（MSC）中的表达水平。采集MDS的骨髓标本，分离、培养和扩增MSC，观察细胞形态并进行表型鉴定；以实时定量RT-PCR法检测间充质干细胞SDF-1基因和内参照磷酸甘油醛脱氢酶（GAPDH）基因的表达水平，并与健康供者的表达水平相比较。结果发现，MDS患者骨髓间充质干细胞SDF-1基因的表达水平经GAPDH校正后为1.53±0.92，与对照组(5.51±0.99)相比差异非常显著(Plt;0.01)。结论：SDF-1基因在MDS患者骨髓间充质干细胞中的表达显著升高，SDF-1基因的异常表达可能影响MDS患者骨髓微环境的造血调控作用，是否可对MDS的发病机制及治疗提供新的思路值得进一步探讨。 【关键词】 骨髓增生异常综合征 　　Expression of SDF-1 Gene in Bone Marrow Mesenchymal Stem Cells of Patients with Myelodysplastic Syndrome 　　Abstract This study was aimed to investigate the expression level of stromal cell derived factor-1 gene (SDF-1) in bone marrow mesenchymal stem cells (MSC) of patients with myelodysplastic syndrome (MDS).The MSC from bone marrow samples of MDS patients were isolated，cultured and expanded，the morphology and immunophenotype of MSC were analyzed.The expression levels of SDF-1 and internal reference GAPDH in MSC of MDS patients were detected by real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) method and were compared with expression levels of healthy donors. The results showed that the expression levels of SDF-1 in MDS patients were significantly different from those in healthy donors (1.53?.92 vs 5.51?.99) (Plt;0.01). SDF-1 gene expression levels in bone marrow MSC of MDS patients were significantly higher than that in MSC derived from healthy donors.It is concluded that the abnormal expression of SDF-1 gene in MSC may influence the regulation of hematopoiesis of the bone marrow microenvironment in MDS patients and it is worthy of further investigation for new clue on etiological mechanism and treatment of MDS. 　　Key words myelodysplastic syndrome; mesenchymal stem cell; SDF-1 gene; real-time quantitative RT-PCR 　　骨髓增生异常综合征 (myelodysplastic syndromes，MDS) 是一组以血细胞的质和量异常为特征的血液系统异质性疾病，迄今为止发病机制尚不明确。多数研究者认为，MDS由造血干细胞的恶性克隆性增殖引起，关于骨髓造血微环境是否参与其发病目前还存在争论［1］。间充质干细胞（mesenchymal stem cell，MSC）是骨髓造血微环境的重