虎杖苷对烧伤血清和lps作用下大鼠平滑肌细胞蛋白激酶c活性的影响.docVIP

虎杖苷对烧伤血清和LPS作用下大鼠平滑肌细胞蛋白激酶C活性的影响 作者：王月刚，金春华，黄海潇，吴平生 【关键词】 虎杖苷 基金项目：国家自然科学基金 【Abstract】 AIM: To explore the mechanism of polydatin (PD) treating shock through studying the influence of PD on the activity of protein kinase C (PKC) of vascular smooth muscle cells (VSMC) when treated by sera from burned rats and lipopolysaccharide (LPS). METHODS: After VSMC was treated by sera from burned rats or LPS, the activity of PKC was measured by γscintillation counting instrument. RESULTS: Activity of PKC of VSMC cytoplasm after treated by sera from burned rats decreased (Plt;0.05 vs normal group), but activity of PKC of cell membrane increased (Plt;0.05 vs normal group). When VSMC was treated with PD, the activity of PKC of VSMC cytoplasm increased and that of cell membrane decreased (Plt;0.05 vs burned group and control group, Pgt;0.05 vs normal group). However, the activity of PKC of VSMC cytoplasm and cell membrane treated by LPS increased (Plt;0.05 vs normal group), after PD treated VSMC the activity of PKC decreased again (Plt;0.05 vs LPS group and control group, Pgt;0.05 vs normal group). CONCLUSION: PD antagonizes the damage effects of LPS and sera of burned rats by reversing the PKC activities, which may be one of the molecular mechanisms of PD antishock property. 【Keywords】 burn; lipopolysaccharide; smooth muscle cell; polydatin; protein kinase C 【摘要】 目的:观察虎杖苷(PD)对烧伤血清和脂多糖（LPS）作用下大鼠血管平滑肌细胞（VSMC）蛋白激酶C（PKC）活性的影响，探讨其抗休克的机制. 方法:取大鼠胸主动脉培养VSMC，用烧伤血清和LPS刺激VSMC后，γ闪烁计数仪测定PKC活性. 结果： 烧伤血清作用后VSMC胞质PKC的活性下降(vs正常组Plt;0.05)，但胞膜的PKC活性上升（vs正常组Plt;0.05），经PD治疗后胞质PKC的活性上升（vs烧伤组、治疗对照组Plt;0.05，vs正常组Pgt;0.05），胞膜的PKC活性下降（vs烧伤组、治疗对照组Plt;0.05, vs正常组Pgt;0.05）. LPS刺激后VSMC胞质与胞膜PKC的活性均上升（vs正常组Plt;0.05），经PD处理后其活性又均下降（vs LPS组、治疗对照组Plt;0.05，vs正常组Pgt;0.05）. 结论： PD对烧伤血清和LPS作用下的VSMC的保护作用可能是通过调节PKC的活性来发挥作用的，这可能是其抗休克的分子机制之一. 【关键词】 烧伤；脂多糖；平滑肌细胞；虎杖苷；蛋白激酶C 0引言 虎杖苷(polydatin, PD)是从中药虎杖中提取的主要成份，在体动物实验表明PD对烧伤性、感染性休克有良好疗效，治疗休克动物的存活时间和存活率均明显优于6542和多巴胺［1］. 平滑肌细胞(