黄芪雷公藤合剂对链脲菌素诱导的糖尿病大鼠肾损伤的防治效果.docVIP

黄芪雷公藤合剂对链脲菌素诱导的糖尿病大鼠肾损伤的防治效果 作者：涂晓 程晓霞 陈洪宇 姜聪娇 【摘要】 ［目的］研究黄芪雷公藤合剂对糖尿病(DM)大鼠肾损伤的治疗作用。［方法］DM模型大鼠分为正常对照组，空白对照组，科素亚治疗组，雷公藤水煎剂治疗组，黄芪雷公藤合剂低、中、高剂量组，除正常对照组外其余各组依次分别给予自来水,科素亚，雷公藤水煎剂，低、中、高剂量黄芪雷公藤合剂,对比观察尿蛋白排泄,血肌酐,血总胆固醇。［结果］黄芪雷公藤合剂能降低实验大鼠的尿蛋白和血肌酐，中大剂量的黄芪雷公藤合剂的降尿蛋白及降血肌酐作用优于科素亚及雷公藤水煎剂，并在改善DN大鼠的脂代谢紊乱方面作用明显优于科素亚。［结论］黄芪雷公藤合剂对STZ诱导DM大鼠肾损伤具有防治作用。 【关键词】 黄芪雷公藤合剂；糖尿病；肾损伤；大鼠 　　Abstract：［Objective］ To investigate the effects of the radix astragalus and tripterygiumforrestii compound on the nephropathy damages in diabetic rats.［Methods］ All the rats were induced by injecting streptozotocin(STZ) into abdomen,and were divided into seven groups： normal control group，model group，western medicine group(treated with Lossartan Potassium Tablets),Chinese herbs control group(treated with tripterygiumforrestii)，high dose treatment group (HDT group)，medium dose treatment group (MDT group)，low dose treatment group (LDT group).The urine protein，serum creatine and cholesterol were tested and compared among the groups.［Result］ The radix astragalus and tripterygiumforrestii compound has the function of reducing the level of urine protein and serum creatine in rats.The functions of MDT group and HDT group are better than western medicine group and Chinese herbs control group in reducing urine protein and serum creatine and they play significantly better than losartan in regulating the lipid metabolic disorder in rats.［Conclusion］The radix astragalus and tripterygiumforrestii compound has positive effect in treating the nephropathy damages in diabetic rats induced by STZ. 　　Key words:radix astragalus and tripterygiumforrestii compound;diabetes;nephropathy damages;rats 　　糖尿病肾病(DN)是糖尿病常见的微血管并发症,亦是终末期肾病的重要原因。糖尿病肾病的发病机理是多因素的，其中糖代谢异常，血流动力学改变以及基因背景是目前认为比较重要的环节。 　　1 材料与方法 　　1.1 材料 黄芪雷公藤合剂由黄芪、雷公藤（去皮饮片久煎）、制首乌、丹参组成，按体重折算分低、中、高剂量。雷公藤水煎剂由本院制剂室提供。科素亚为默沙东公司生产。链脲菌素(STZ) 购自美国Sigma 公司，以0.1mol/L,PH 4.2枸橼酸缓冲液溶解，新鲜配制。动物系SD雄性大鼠，体重200～ 220g，浙江中医药大学动物实验中心提供。 　　1.2 方法 　　1.2.1 动物造模及处