胶质细胞活化对慢性前列腺炎-慢性盆腔疼痛综合征(cp-cpps)大鼠脊髓背角p物质的影响.docVIP

胶质细胞活化对慢性前列腺炎/慢性盆腔疼痛综合征(CP/CPPS)大鼠脊髓背角P物质的影响 【摘要】 　　目的 探讨胶质细胞活化对慢性前列腺炎/慢性盆腔疼痛综合征(chronic prostatitis/chronic pelvic pain syndromes, CP/CPPS)大鼠脊髓背角P物质的影响。方法 完全福氏佐剂和3%角叉菜胶前列腺内注射造成CP/CPPS模型，脊髓插管给药胶质细胞活化抑制剂Propentofylline干扰CP/CPPS模型大鼠，免疫组织化学方法观察正常组、疼痛模型组、药物干扰组脊髓节段(L6和S1)背角的胶质细胞的活化和P物质的定性定位，并用放射免疫的方法观察三组脊髓背角P物质的变化。结果 脊髓背角胶质细胞活化阳性细胞数疼痛组明显增加，药物干扰组明显减少；P物质主要表达于脊髓背角且疼痛模型组明显增多，药物干扰组明显减少。结论 胶质细胞活化是CP/CPPS大鼠脊髓背角P物质变化的重要原因，胶质细胞活化抑制剂的应用将是治疗CP/CPPS的新亮点。 【关键词】 前列腺炎　疼痛　P物质　胶质细胞 　　Substance P of cornu dorsale medullae spinalis affected by activation of glia in rats with chronic prostatitis / chronic pelvic pain syndromes 　　ABSTRACT: Objective To explore the relationship between activation of glia and substance P of cornu dorsale medullae spinalis in rats with chronic prostatitis/chronic pelvic pain syndromes (CP/CPPS). Methods The models of CP/CPPS were induced by injection of complete Freund s adjuvant and 3% carrageenan in prostate. Propentofylline was given with PE10 in spinal cord of rat models. The activation of glia and substance P in the spinal cord was detected with immunohistochemistry and the change of substance P was observed by radioimmunity. Results Activation of glia was significantly increased in models and significantly reduced in interfered groups, and substance P was significantly increased in models and significantly reduced in the interfered groups. Conclusion The activation of glia in the spinal cord was one important reason of the change in substance P excreted from cornu dorsale medullae spinalis. Inhibitor of glia activation will be a new way to treat CP/CPPS. 　　KEY WORDS: prostatitis; pain; substance P; glia 慢性前列腺炎/慢性盆腔疼痛综合征(chronic prostatitis/chronic pelvic pain syndromes, CP/CPPS)是泌尿门诊的常见病，疼痛是患者就诊的主要原因，严重影响了患者的生活质量，所以对慢性前列腺炎患者疼痛机制的探讨尤为重要，但国内外在这方面研究并不多。P物质(substance P, SP)是中枢神经系统内疼痛的重要调质和递质，它被认为是脊髓伤害性信息的传递物质，在脊髓痛觉信息传递过程中发挥着重要的作用，而越来越多的研究发现胶质细胞，主要是星形胶质细胞和小胶质细胞，不仅仅起支持和营养作用，还参与中枢疼痛的调控，所以本实验旨在观察CP/CPPS大鼠模型中胶质细胞活化