高密度Oligo基因芯片筛选小鼠前脑发育过程中基因表达的研究.docVIP

Screen the gene expression profiles of the developmental mice forebrain by large-scale Oligo microarray technique Abstract The major interneurons in the olfactory bulb(OB), the periglomerular and granule cells, are derived from neural stem cells (NSCs) that migrate from the lateral ganglionic eminences(LGE) in embryo. Postnatally, they are derived from NSCs that migrate in the rostral migratory stream(RMS) from the anterior subvenricular zone (SVZa) in the lateral ventricles of the forebrain. The SVZa-derived cells, after arriving at the OB, disperse radially and ultimately differentiate into the granule and periglomerular cells of the OB. This form of migration is classified as chain migration. It was found that about 70% and 30% of periglomerular interneurons were immunoreactive for GABA and tyrosine hydroxylase (TH) respectively. Local environments have an important role in the proliferation, migration and differentiation of the SVZa-derived cells and numerous genes may be involved in this process. DNA microarray technique is an advanced molecular biological platform which can profile the dynamic changes of more than ten thousands of genes. In this study, a total of 12 RNA samples from different regions of OB, RMS and SVZa in the four different stages( Embryonic day 16 (E16), Postnatal day 1(P1), P7 and P21) were screened by large-scale Oligo microarray to identify genes related to chain migration during mice forebrain development. The main results were summed up as follows. 1.DNA microarray experiment We used oligo microarrays that were purchased from Capital Biochip Corporation National Engineering Research Center for Beijing Biochip Technology and contain probes for 16,844 genes to profile comprehensive molecular and genetic programs underlying the developmental mice forebrain. Microarray experiment design involved the preparation of the total RNA samples. Twelve experimental samples were obtained and pooled from the same regions and time points in differe