改过PreparationandResearchofPorousHydroxyapatiteWhiskersandKGMCompositeBoneScaffolds.docVIP

Preparation and Research of Porous Hydroxyapatite Whiskers/KGM Composite Bone Scaffolds Huang Minghua1, a, Chen Qinghua2, b, Lei Li2, c, Wang Duancheng2, d and Yan Tingting2, e 1Faculty of Mechanical and Electrical Engineering of Kunming University of Science and Technology, Kunming, 650500, China 2Faculty of Materials Science and Engineering of Kunming University of Science and Technology, Kunming, 650500, China ahuangminghuajj@, bchenqinghua_yn1@163.com, ckmust2007ll@, d625296056@, ettyan@ Keywords: Hydroxyapatite Whisker (HAPw), Konjac Glucomannan (KGM), Crosslinking Agent, Freeze-Drying Method, Porosity Abstract. Sol-gel method and freeze-drying method were adopted to prepare the porous HAPw/KGM composite bone scaffolds and ammonia was used as a crosslinking agent. The porosity, average pore diameter, compressive strength and degradation rate in vitro were measured according to the related standard. The curves of each factor and lever affecting comprehensive properties were drew through the orthogonal design L9 (34) experiment. SEM and XRD were applied in characterization. The results show that the optimal preparation program of the composite scaffolds is KGM (2g), HAPw (4.5g), ammonia (0.1 ml) and the freeze temperature (-20 ° C); the prepared scaffolds are porous three-dimensional network structures; the porosity of optimal scaffold is more than 90%; the average pore diameter is between 200-300μm; the compressive strength is about 0.8Mpa and the degradation rate is about 50% within 9 weeks. Introduction At present, the bone scaffolds and the products of bone tissue engineering are still in the stage of basic research and there exists a larger gap away from the requirements and standards of clinical applications. Research and development of the ideal bone scaffolds and the products of bone tissue engineering, which can effectively promote formation of new bones and complete regeneration of bone tissue, becomes focus and difficulties. For bone tissue eng