基础研究appswepse9mirna-j.docVIP

· · 1280 南方医科大学学报（J South Med Univ） 2012;32(9) 基础研究 APP swe/PS△ E9双转基因小鼠脑组织miRNA的表达 丁 宇，田 密，刘建锋，邓炎尧，李 维，奉夏露，侯德仁 中南大学湘雅三医院神经内科，湖南 长沙 410013 摘要：目的 观察APPswe/PS△ E9双转基因小鼠与正常小鼠脑组织内miRNA的差异表达，探索miRNA在阿尔茨海默病中的可 能作用。方法 采用6月龄APPswe/PS△ E9双转基因小鼠作为实验组，同月龄、同种系野生型小鼠C57作为对照组，基因芯片检 测两组小鼠脑组织miRNA表达；比较两组小鼠miRNA的差异表达。结果 与对照组比较，实验组中表达上/下调2倍以上的 miRNA 有 miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA-466b-3p、miR-669-3p、miR-142-5p、miR-144、miR-466f-3p、 miR-466g、miR-200a、miR-200b、miR-96 等 12 种 ，但 有 统 计 学 意 义（P0.05）的 均 是 下 调 的 5 种 miRNA：miRNA-135a、 miRNA-135a-2*、miRNA-298、miRNA-466b-3p 和 miR-669-3p。 结 论 miRNA-135a、miRNA-135a-2*、miRNA-298、miRNA- 466b-3p和miR-669-3p可能是在APPswe/PS△ E9双转基因小鼠发病中有意义的miRNA。 关键词：APPswe/PS△ E9双转基因小鼠；Alzheimer 病；miRNA；基因芯片 中图分类号：R34 文献标志码：A 文章编号：1673-4254（2012）09-1280-04 doi: 10.3969/j.issn.1673-4254.2012.09.013 /kcms/detail/44.1627.R0907.004.html Expression profile of miRNAs in APP swe/PS△E9 transgenic mice DING Yu, TIAN Mi, LIU Jianfeng, DENG Yanyao, LI Wei, FENG Xialu, HOU Deren Department of Neurology, Third Xiangya Hospital, Central South University, Changsha 410013, China Abstract: Objective To observe the changes of miRNA expression profiles in APPswe/PS△E9 transgenic mice and explore the possible roles of miRNA in the pathogenesis of Alzheimer’s disease. Methods Using miRNA chip technique, we examined the miRNA expression in the brain tissue of 6-month-old APPswe/PS△E9 transgenic mice, with age-matched wild-type mice as the control group. Results Twelve miRNAs showed differential expressions by more than two folds in APPswe/PS △ E9 transgenic mice, namely miRNA-135a, miRNA-135a-2*, miRNA-298, miRNA-466b-3p, miR-669-3p, miR-142-5p, miR-144, miR-466f-3p, miR-466g, miR-200a, miR-200b and miR-96. Five miRNAs were significantly down-regulated in the transgenic mice, including miRNA-135a, miRNA-135a-2*, miRNA-298, miRNA-466b-3p, and miR-669-3p. Conclusion The 5 down- regulated miRNA may play important roles in the pathogenesis of AD in APPswe/PS△E9 transgenic mi