QCM General presentation培训教程文件.pptVIP

Q-Sense, G?teborg, Sweden QCM ? QCM-D ? Q-Sense 1960s: QCM for monitoring of thin films in air and vacuum 1972 QCM as biosensor 1980s QCM is operated in liquid 1990s QCM is further developed into QCM-D 1995 QCM-D technology patented 1996 Q-Sense founded 1999 1st QCM-D generation launched 2005 2nd QCM-D generation launched 2008 250 users in 30 countries 30 companies (Amgen, SmithNephew, Nippon Paper, JohnsonJohnson, Colgate/Palmolive, PG, Unilever...) 350 publications Common application areas What can QCM-D characterise? Cell/protein attractive surfaces Protein resistant surfaces Sensing the environment Enzymatic Removal of Model Stains Nanotechnology QCM-D ping principle Data acquisition Data output from QCM-D Q-Sense System Overview Electronics unit Measurement chamber Flow modules Sensor crystals Software Q-Sense E4 Q-Sense E4 Q-Sense E4 features and benefits 4 sensors in separate flow modules Sensitivity, 0.5 ng/cm2 Measurement rate, 100 points/s Removable flow modules Controlled one-direction flow Volume above sensor ~ 40 μL Sample volume ~ 300 μL Quick, precise temperature control High frequency capacity, 70 MHz Chemical compatibility HPLC fittings Standard flow module Standard flow module Surfaces supplied by Q-Sense QuantificationEnzymatic cleavage of antibodies Sensing conformational changes Crosslinking Insert crosslinking animation here! Analyzing cleaning degradation Insert cleaning animation here! Follow break-up of films, mass removal, swelling, desorption rates… Three commercially available kitchen spray cleaners treating a sensor coated with a model stain. Surfactant Removal of Model Stains Temperature concentration effects on cleaning efficiency 1 component system using single surfactant (TritonX-100) Removal of greasy stain (triolein) measured at 25 C and 40 C Removal of greasy stain (triolein) measured at 3 different concentrations Enzymatic degradation of lipids Enzymatic degradation of lipids Enzymatic degradation of lipids