校准品的复溶及校准次数对生化测量系统校准的影响.docVIP

校准品的复溶及校准次数对生化测量系统校准的影响 东台市人民医院 林素萍 臧素纲 韩霜 周玉贵 摘要： 目的 探讨生化测量系统校准过程中校准品复溶方法及校准次数对校准的影响。方法 校准品的复溶采用称重复溶法、定量移液管法及普通吸管法等三种方法，校准进行单次校准和多次校准取平均值，分别对ALT、AST等十二个项目进行校准和验证试验。结果 定量移液管加样法校准的平均K值与称重加样法校准的平均K值比较接近，普通吸管加样法校准的平均K值与称重加样法校准的平均K值的偏移较大，单次校准的K值之间有明显差异，多次校准的平均K值赋予生化仪进行的验证结果更接近用于被验证的校准品相应项目的靶值。结论 定量移液管复溶法可作为实验室常规复溶方法，同时建议生化测量系统应用多次校准的平均K值进行测量。 关键词：生化测量系统；校准品；校准; 平均K值 The influence of dissolving calibrator and calibration times to calibration of biochemical measurement system Abstract: Objective to investigate the influence of dissolving calibrator and calibration times for the biochemical measurement system. Methods respectively dissolving calibrator by three methods with balance weighing mass, volumetric pipette and ordinary straw, calibration ways include single calibration and calculating average after repeatedly calibrating five times, respectively calibrating validation test for twelve items ,such as ALT, AST, etc. Result the average K value of volumetric pipette method is very close to the average K value of balance weighing method, there is obvious difference in the average K value between ordinary straw method and balance weighing method, the K values of calibration are obvious different each time. we give the average value of calibration to the biochemical analyzer , and proceed validation test. We find the results of validation test is more coincide to the target value of the calibrator which manufacturers provided. Conclusion volumetric pipette method can be used as the routine method for dissolving calibrator in laboratory, and we suggest to calculate the average K value of calibration and give the average value of calibration to biochemical measure system. Key words: biochemical measurement system ; calibrator ; calibration; average K value 生化分析仪检测结果的准确性直接影响到临床对患者的诊治。正确地实施测量系统的校准是保证生化项目检测准确性的前提，也是临床工作者需解决的当务之急。校准是在规定条件下，确定测量仪器、测量系统的示值或实物量具所代表的值与相应的被测量的已知值之间关系的一组操作[1-2]。测量仪器校准是一个或多个辅助设备组合，用于对测量的装置进行校准，分为质检部门检定和厂方校准。测量系统校准是对一套组