FcαR1基因5′端调控及UTR区SNPs及其单倍型与华南地区汉族IgA肾病患者预后关系.docVIP

FcαR1基因 5′端调控及UTR区SNPs及其单倍型与华南地区汉族IgA肾病患者预后关系 　　作者：陈伟强,古宏标,李幼姬,黄玮俊　　作者单位：广东药学院 基础学院，广东 广州 　　【摘要】目的 研究我国南方汉族IgA 肾病患者FcΑR1基因5′端调控及UTR区SNPs及其单倍型与我国南方IgA肾病患者预后的关系。方法 采集266例肾活检证实的IgA肾病患者血样，提取基因组DNA。用PCR产物直接测序法鉴定基因型，每3～6个月查尿蛋白、血肌酐及其他指标随访，以血肌酐浓度比基础值升高1倍以上或死亡作为随访终点。采用单因素相关分析及Logistic多元回归分析各位点多态性及其单倍型与肾功能恶化及预后的关系。结果 (1)该区域单倍型对及-27、56位点多态性与肾功能进展显著相关;(2) -27位点基因型TC与TT比较差异显著(P=0.003); 56位点基因型CC与TT比较，P=0.011;单倍型对TCCCTC与TTTTTT比较，P=0.000。结论 FcΑR1基因第一外显子C56T多态位点基因型与我国南方IgAN患者预后相关。 　　【关键词】 IgA肾病; FcΑR1基因;多态性; 预后;相关分析 　　SNPs and it′s haplotypes of FcΑR1 gene in 5′ regulatory region and UTR region were associated with the prognoses of Chinese Han IgA nephropathy (IgAN ) patients in the South China 　　CHEN Weiqiang， GU Hongbiao， LI Youji，HUANG Weijun 　　1.School of Foundation Studies, Guangdong Pharmaceutical University, Guangzhou,Guangdong 510006; 　　2.Department of Nephrology, First Affiliated Hospital, SunYatsen University, Guangzhou,Guangdong 510080; 　　3.Department of Medical Genetics, Zhongshan School of Medicine, Sun Yatsen University, Guangzhou,Guangdong 510089,China 　　AbstractObjective To investigate the relationship between the prognoses and the polymorphisms of FcΑR1 gene in Chinese Han IgA nephropathy (IgAN) patients in the South China.Methods 266 blood samples from renalbiopsies that had been proven IgA nephropathy were collected，the genotypes were determined by PCR product sequencing.All patients were followed up every 3 to 6 months by examining urinary protein, Scr, and other indicators.Followup was terminated when Scr value increased by one fold than the normal or in the case of the death of the patients.Followup lasted for more than six months.The relationship between the renal function and the polymorphisms of FcΑR1 gene were evaluated by single factor analysis and multiple regression analysis with SPSS 10.0.Results (1)Haplotypes and -27, 56 loci were significantly correlated with the aggravation of renal function.(2)Genotype TC