silencingofbidirectionalpromotersbydnamethylationin.ppt

Silencing of Bidirectional Promoters by DNA Methylation in Tumorigenesis by Jingmin Shu, Jaroslav Jelinek, Hao Chang, Lanlan Shen, Taichun Qin, Woonbok Chung, Yasuhiro Oki, and Jean-Pierre J. Issa Cancer Res Volume 66(10):5077-5084 May 15, 2006 ?2006 by American Association for Cancer Research Schematic illustration of three bidirectional promoters: (A) WNT9A/CD558500 promoter, (B) CTDSPL/BC040563 promoter, (C) KCNK15/BF195580 promoter. Jingmin Shu et al. Cancer Res 2006;66:5077-5084 ?2006 by American Association for Cancer Research Bisulfite sequencing in normal control and cell lines. Jingmin Shu et al. Cancer Res 2006;66:5077-5084 ?2006 by American Association for Cancer Research Expression levels of three bidirectional gene pairs. Jingmin Shu et al. Cancer Res 2006;66:5077-5084 ?2006 by American Association for Cancer Research WNT9A/CD558500 promoter is bidirectional. Jingmin Shu et al. Cancer Res 2006;66:5077-5084 ?2006 by American Association for Cancer Research Expression level of bidirectional gene pairs in unmethylated or hypermethylated ALL patient samples. Jingmin Shu et al. Cancer Res 2006;66:5077-5084 ?2006 by American Association for Cancer Research Schematic illustration of three bidirectional promoters: (A) WNT9A/CD558500 promoter, (B) CTDSPL/BC040563 promoter, (C) KCNK15/BF195580 promoter. Arrows, direction of gene transcription. Vertical bars, CpG sites. Open boxes, first exons of each gene. Gray areas, range tested by pyrosequencing or COBRA for DNA methylation. Thick black lines, range tested by bisulfite sequencing. R, 5′-end of RACE PCR products. Bisulfite sequencing in normal control and cell lines. A, WNT9A/CD558500 promoter in normal control, RAJI, and one ALL patient sample. B, CTDSPL/BC040563 promoter in normal control, HL60, and one ALL patient sample. C, KCNK15/BF195580 promoter in normal control, SW48, and one ALL patient sample. Circle, CpG site. Open circles, unmethylated CpG sites. Solid circles, methylated CpG sites. Row, indiv