刺梨论文刺梨水提物抗氧化损伤作用研究.docVIP

刺梨论文：刺梨水提物抗氧化损伤作用研究 【中文摘要】氧化损伤会导致器官和组织病变,诱发多种疾病如癌症、老化、动脉粥样硬化、糖尿病以及类风湿性关节炎等。刺梨（ Rose roxburghii Tratt）自然资源丰富,天然、无毒、富含多种营养成分如Vc、超氧化物歧化酶（SOD）、刺梨黄酮、刺梨多糖、人体必须氨基酸等并具有多种功能活性。本试验通过多个体系测定刺梨水提物的抗氧化损伤作用。结果证明刺梨水提物具有较好的抗氧化损伤能力。刺梨水提物中多酚类化合物的含量223.02±12.89 mg/g,黄酮类化合物含量20.41±1.71 mg/g。刺梨水提物对DPPH·自由基、ABTS+·自由基均有较强的清除能力,其清除DPPH·自由基的IC50值为74.93 mg/L,清除ABTS+·自由基的IC50值为153.83 mg/L。刺梨水提物也具有较强的铁 【英文摘要】Oxidative damage can lead to organ and tissue lesions and induce a variety of diseases such as cancer, aging, atherosclerosis, diabetes, and rheumatoid arthritis etc.Rose roxburghii Tratt which is rich in natural resources, is non-toxic and full of nutrients, such as Vc, superoxide dismutase （SOD）, flavone, polysaccharides, essential amino acids etc. Therefore, it has a variety of functional activity.In this paper, multiple reaction systems were used to determine the effect on oxidative damage of aqueous extract of Rose roxburghii Tratt.In aqueous extract of Rose roxburghii Tratt, polyphenols content was 223.02?2.89 mg/g, flavonoid content was 20.41?.71 mg/g. The Rose roxburghii Tratt aqueous extract had scavenging activity on DPPH穜adical and ABTS+穜adical. Its IC50 was 74.93 mg/L on scavenging DPPH穜adical and IC50 was 153.83 mg/L on ABTS+穜adical. Rose roxburghii Tratt aqueous extract also had strong Iron reductive ability, the FRAP was 1.363 mmol。The inhibitory action on induced DNA oxidative damage by Phen-CuSO4-Vc system was enhanced with concentration of the Rose roxburghii Tratt aqueous extract increased. When concentration reached 15mg/ml, the inhibitory rate was 66.9%.Rose roxburghii Tratt aqueous extract had inhibitory in Cu2+/H2O2 and AAPH induced protein oxidative damage. When concentration reached 10mg/ml, protein oxidative damdge was inhibited significantly。Rose roxburghii Tratt aqueous extract had protection effect on Cu2+/H2O2 induced liver homogenate protein oxidative damage. It could inhibit protein carbonyls. The inhibitory effect was concentration depedent. It could