复方参七汤对胃结直肠癌抑制作用的实验研究.docVIP

复方参七汤对胃、结直肠癌抑制作用的实验研究 　　作者：钱晶晶，沈洪薰，陆 飞，周树军，陈玉泉 作者单位：南通大学附属医院普外科， 南通226001 　　【摘要】目的：研究复方参七汤治疗胃、结直肠癌的作用机制。方法：(1)复方参七汤对人胃癌细胞的体外抑制实验;(2)对MNNG诱发大鼠腺胃癌的体内抑制诱癌率实验;(3)对裸鼠结肠癌肝转移的体内抑制实验。结果：(1)复方参七汤对人胃癌BGC-823细胞株半数杀伤浓度(LC50)为0.048 g/ml，随药物浓度增高，杀伤作用亦增强;半数抑制浓度(IC50)为0.007 g/ml，随药物浓度增高，抑制有丝分裂指数逐渐减小，而抑制细胞集落形成亦增大;(2)复方参七汤对MNNG诱发大鼠腺胃癌发生率明显降低，实验组为6.9%(3/43)显著低于对照组58.9%(23/39)，(P0.01);对MNNG诱发大鼠腺胃癌组织中PCNA和CyclinD1阳性表达明显降低，实验组为13.88%(5/36)和22.86%(8/35)，显著低于对照组75.76%(25/33)和62.86%(22/35)，(P0.01);(3)复方参七汤对人结肠癌LS174t细胞株接种裸鼠肝转移的肝转移数、肝转移癌重和肝癌重/肝重明显降低，实验组为2.42±0.99，63.67±21.29和8.90±3.10，显著低于对照组4.17±1.99，94.32±37.86和13.20±5.30(P0.05);对裸鼠肝转移血中CK20 mRNA表达的相对值减少，实验组为0.398±0.143显著低于对照组0.518±0.090，(P0.05);对裸鼠肝转移组织中nm23H1-NDPK阳性表达呈过表达，实验组为66.67%(8/12)显著高于对照组16.6%(2/12)，(P0.05)。结论：复方参七汤在体外实验有杀伤癌细胞作用;在体内实验抑制大鼠对MNNG诱癌率;抑制裸鼠对人结肠癌细胞肝转移的作用。 　　【关键词】 胃癌 复方参七汤 肝转移 　　Experimental study of inhibitory effect of compound Shenqitang on gastric and colorectal carcinomas 　　QIAN Jingjing, SHEN Hongxun, LU Fei, et al (Department of General Surgery, Affiliated Hospital of Nantong University, Nantong 226001) 　　[Abstract] Objective: To investigate the mechanism of therapeutic effect of compound Shenqitang on the gastric and colorectal carcinomas. Methods: Several experiments had been done, including:(1) the inhibitory effect of compound Shenqitang on human gastric adenocarcinoma cell line in vitro;(2) its inhibitory effects on Wistar rat gastric adenocarcinoma induced by MNNG; and (3) its inhibitory effect on the hepatic metastases of colorectal carcinoma in mice. Results: (1)Obvious inhibitory effects of compound Shenqitang had been found on the cell division and colony form of human gastric adenocarcinoma cell line BGC-823 in vitro. The LC50 was 0.007g crude drug/ml in colony form experiment. The drug had potent killing effect in higher concentration, with LC50 of 0.048g crude drug/ml;(2)The incidence of gastric adenocarcinoma in the experimental group(6.9%) was significantly lower than that in t