情志刺激法建立肝郁脾虚模型的研究.docVIP

情志刺激致大鼠肝郁脾虚证模型的研究 郭建丽 冯玛莉* （山西省中医药研究院，山西 太原 030012） 复合病因大鼠造模法：大鼠钢丝网笼中令其不能自由活动每日束缚6h每笼一只每周不定时的禁食两次3周正常一笼合养 关键词：情志刺激；肝郁脾虚；模型 Old stimulation method to establish the model of the spleen deficiency stomach GUOJian-li, FEN Ma-li (Chinese Medicine Institute of Shanxi, Taiyuan, 030012, China) Abstract: Objective The chronic bound + diet dishonor of a solitary + method copy the spleen deficiency stomach model, for its further development and research to provide the scientific basis Methods Male and female mice half and half,, it will be randomly divided into blank control group, model control group, free and unfettered scattered group, a tonic diarrhea liver soup groups, and the only 10.Composite causes own rat made mode method: will build model rats in homemade steel wire WangLong, make its body and limbs not free activities, normal group only a cage $5 a, model control group, free and unfettered scattered group, a tonic liver soup group diarrhea, laboratory mice every cage a solitary raise, daily bound 6 h, from 9 am-3 PM. A week of rats timing fast twice, time of the mould made 3 weeks.Results And blank compared to the model control group of rats weight a slow, sugar water consumption is low, the horizontal movement and vertical motion in the water, reduce static time increases, the thymus and spleen index index is low, free and unfettered scattered to the rat of symptoms were obviously improved, purging tonic liver group has certain effect.Conclusions Establishment of TCM syndrome with characteristics of the spleen deficiency stomach model has an important meaning, and through the chronic bound + diet dishonor of a solitary + method can copy the spleen deficiency stomach successful model. KEY WORDS: Sentiment will stimulate;Spleen deficiency stomach;model 肝郁脾虚证是中医脏腑最常见的病理证候之一。据统计，中医五藏中发病率最高的是肝脏——占五藏发病的39.6％，41.9%[1]。肝郁脾虚证的基本病变是肝失疏泄，脾失健运，主要表现为：精神抑郁，情绪不宁、胸胁胀闷或易怒善哭、脘闷嗳气，不思饮食，大便不调为特征的一类临床病证，其形成多与现代抑郁、焦虑、悲痛等负面情绪心理应激状态有关。从现代医学观点来看，涉及神经、消化、免疫等多系统的功能改变。本实验模拟现代人的情志不遂致病的病因病