Genomic组织和染色体的小鼠附睾视黄酸结合蛋白基因.docVIP

Genomic组织和染色体的小鼠附睾视黄酸结合蛋白基因（ME-RABP）定位。 Abstract 文摘 The murine epididymal retinoic acid-binding protein (mE-RABP) is specifically synthesized in the mouse mid/distal caput epididymidis and secreted in the lumen. 鼠类附睾的视黄酸结合蛋白(mE-RABP)是专门合成在老鼠中/远端头epididymidis和中分泌腔。 In this report, we have demonstrated by Southern blot analysis of genomic DNA that mE-RABP is encoded by a single-copy gene. 在这篇报告中,我们已经演示了由南方污点,mE-RABP基因组DNA的分析是由基因进行编码的单份。 A mouse 129/SvJ genomic bacterial artificial chromosome (BAC) library was screened using a cDNA encoding the minor form of mE-RABP. 一只老鼠129 / SvJ基因组的细菌人工染色体(BAC)库是使用一个cDNA筛选编码小mE-RABP形式。 One positive BAC clone was characterized and sequenced to determine the nucleotide sequence of the entire mE-RABP gene. 一个积极的BAC克隆特点和测序来确定整个mE-RABP核苷酸序列的基因。 The molecular cloning of the mE-RABP gene completes the characterization of the 20.5-kDa-predicted preprotein leading to the minor and major forms of mE-RABP. 分子克隆的mE-RABP基因完成描述20.5负责预测preprotein导致轻微和主要形式的mE-RABP。 Comparison of the DNA sequence of the promoter and coding regions with that of the rat epididymal secretory protein I (ESP I) gene showed that the mE-RABP gene is the orthologue of the ESP I gene that encodes a rat epididymal retinoic acid-binding protein. 比较DNA序列的发起人和编码区域与鼠附睾的分泌蛋白我(ESP我)基因表明,mE-RABP基因是orthologue的ESP我的编码基因鼠附睾的视黄酸结合蛋白。 Several regulatory elements, including a putative androgen receptor binding site, CACCC-boxes, NF-1, Oct-1, and SP-1 recognition sites, are conserved in the proximal promoter. 一些监管元素,包括一个假定的雄激素受体结合位点,“CACCC-boxes,“nf 1,10月1日,sp 1识别网站,是守恒的近端启动子。 Analysis of the nucleotide sequence of the mE-RABP gene revealed the presence of seven exons and showed that the genomic organization is highly related to other genes encoding lipocalins. 分析mE-RABP核苷酸序列的基因显示存在七个外显子和显示,基因组织是高度相关的其他基因编码lipocalins。 The mE-RABP gene was mapped by fluorescent in situ hybridization to the [A3-B] region of the murine chromosome 2. mE-RABP的基因所绘制荧光原位杂交(a3 b]区域的