黄曲霉毒素的免疫检测方法摘要.ppt

The Immunology-based Methods for Aflatoxins Detection Nutrition and Food Hygiene Ⅰ Classification of immunoassay 1. indirect immunoassay 2. sandwich immunoassay Ⅱ Enzyme-linked immunosorbent assays Enzyme immunoassay is a good alternative to chromatography, because it allows an analysis of massive number of samples, easily automatized and does not require time-consuming procedures and sophisticated equipment. Presently enzyme-linked immunosorbent assay (ELISA) is widely accepted as the‘‘gold standard’’ screening method. In ELISA , horseradish peroxidase (HRP) is commonly used as a label of im