红掌组织培养中不定芽诱导和增殖的研究.docVIP

红掌组织培养中不定芽诱导和增殖的研究 赵斌，李英丽，方正* 河北农业大学 河北省生物无机化学重点实验室，河北 保定 071001) NAA和IBA对红掌组织培养中不定芽诱导和增殖的影响进行研究。得出不定芽诱导和增殖的最佳培养浓度。结果表明，MS培养基对红掌不定芽诱导效果最好，诱导率为85.96%，增殖系数4.12；1.0 mg/L 6-BA为红掌不定芽增殖最佳浓度率IBA 0.3mg/L。 关键词：红掌；组织培养；诱导；增殖 中图分类号： S682.14 文献标识码： Study on the adventitious buds induction and proliferation in Tissue Culture of Anthurium andraeanum ZHAO Bin1, LI Ying-1i1 , FANG Zheng1* (Key Lab of Bio-inorganic Chemistry .Agricultural University of Hebei. Baoding 071001 .China) Abstract： The factors different concentrations of MS medium, cytokinin6-BA, growth hormone NAA and IBA that influence the adventitious buds induction and proliferation in Tissue Culture of Anthurium andraeanum were studied. The optimal culture concentration of proliferation of adventitious buds was be found. The results showed that MS medium was the best for Anthurium adventitious buds and the induction rate was 85.96%, the proliferation coefficient mg / L 6-BA for the Anthurium adventitious buds was the optimal concentrations and the induction rate was 96.3%, proliferation coefficient 6.67. 0.3mg/LIBA for the Anthurium adventitious buds was the optimal concentrations and the induction rate was 96.31%. The most appropriate ratio of MS +6- BA 1.0mg / L + IBA 0.3mg / L. Key Word：Anthurium andraeanum; tissue culture;; 红掌(Antlturium andraeanum )又名安祖花花烛、灯台花、蜡烛花为天南星科花烛属多年生附生常绿草本花卉红掌组织培养技术体系不定芽的诱导和增殖至关重要的作用材料与方法红掌品种红粉佳人(Anthurium andraeanum cv. Sonate) 健康植株的幼嫩叶片为试材直径5mm大小，将整块不定芽按照自然生长状态进行切割，转接在增殖培养基内进行不定芽的增殖培养培养条件温度为25±2℃，光照时间为13h/d，光照强度为1000 Lux-1500 Lux，NAA和IBA、细胞分裂素6-BA为研究对象，设置不同浓度处理，60 d后统计各处理的不定芽诱导率污染率不同培养基对红掌不定芽诱导的影响不同培养基对红掌不定芽诱导1/2 MS、 MS，比较红掌愈伤组织对不定芽的诱导效果。由图1可知，不同浓度培养基对红掌不定芽诱导的影响不同，且各处理间差异显著。其中MS培养基效果最好，使不定芽诱导率达到了85.96%的最高值，有效地促进了红掌不定芽的诱导。其次是1/2 MS培养基，其不定芽诱导率为53.49%，能够对不定芽进行诱导但效果一般。不定芽诱导率最低的为1/4 MS培养基，诱导率仅为36.71%，对不定芽诱导效果最差。 图1 不同培养基对红掌不定芽诱导的影响 Fig. 1 Effects of different culture medium on the induction of adventitious buds 注：图中小写字母表示方差分析差异显著性（P0.05）不同培养基对红掌不定芽以MS、1/2 MS、1/4 MS培