芦笋多糖对S180小鼠红细胞CR1(CD35)数量及活性的影响.docVIP

芦笋多糖对S180小鼠红细胞CR1数量及活性的影响摘要：目的 探讨芦笋多糖对S180小鼠红细胞CR1数量、红细胞的。方法 取小鼠的新鲜红细胞，用大鼠抗小鼠CD35一抗和FITC羊抗大鼠二抗标记小鼠红细胞，应用流式细胞仪测定小鼠红细胞CR1数量；用红细胞免疫花环试验检测红细胞C3b受体花环率(RBC-C3bRR)和肿瘤红细胞细胞花环率(DTER)。结果 芦笋多糖高剂量组和中剂量组使S180小鼠红细胞CR1数量和红细胞C3b受体花环率显著性。结论 芦笋多糖可以改善S180小鼠红细胞免疫功能,可能是其抗肿瘤作用的机制之一。 关键词：芦笋多糖；红细胞；CR1数量；C3b受体花环率；肿瘤红细胞花环率 Effect of Asparagus polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) of S180 mice JI Yu-bin, MIN Yong-cui, JI Chen-feng (Center of Research on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076) Abstract : Objective To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1 in S180 mice. Methods Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of ECR1. Using microscope, we studied the the adherence between erythrocyte immunity and C3b receptor or tumor-cell by RBC-C3bRR and DTER. Results Comparing the mean value of the number of CR1 on each RBC of high and middle groups with control groups , the mean value of the number of CR1,RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups are increased significantly. Conclusion Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice, which may be one of its most important antitumor mechanisms. Key words: Asparagus officinalis polysaccharide; erythrocyte;the number of ECR1 ;RBC-C3b RR; DTER 芦笋(Asparagus officinalis Linn)，学名石刁柏，又名龙须菜?文山竹?索罗罗?细百叶部，是百合科天门冬属多年生草本植物.以往研究表明，其含有皂苷?黄酮?多种氨基酸?多种维生素?硒?锌?铁及蛋自质?糖等多种营养成分，具有防治肿瘤的作用[1]。 红细胞CRl (ECRlerythrocyte complement receptor Type 1,CD35)，即红细胞C3bC4b受体，其基因结构、分子结构以及生物学功能已研究清楚，是红细胞免疫的重要基础。许多实验已证明红细胞CR1分子的数量及其活性变化与免疫相关性疾病、肿瘤及自身免疫性疾病的发生、发展、趋向具有明显的相关性。 [基金项目] 国家自然科学基金项目,哈尔滨市青年基金项目(P260013) [作者简介] *季宇彬,男,1956年生,教授.Tel: (0451) E-mail: jichenfeng@hrbcu.edu.cn. 前期实验芦