高效液相色谱法测定开口箭药材中绿原酸含量.docVIP

高效液相色谱法测定开口箭药材中绿原酸含量 （陕西理工学院 陕西省资源生物重点实验室 陕西汉中 723000）摘 要 目的：建立开口箭中绿原酸含量测定的方法。方法：以50%的甲醇溶液为溶剂，从开口箭药材中提取绿原酸，提取条件为：超声功率240W，超声频率40KHz，提取温度60℃，超声提取30min。采用高效液相色谱法检测供试液中绿原酸含量，色谱条件为：色谱柱为Inertsil ODS-3C18（4.6×150mm，5μm）柱，以乙腈－0.4% 磷酸水溶液（9:91）为流动相，流速1.0mL/min，检测波长327nm，柱温30℃。结果：绿原酸在0.255~3.06μg / mL范围内与峰面积呈现良好的线性关系，Y=31388X+152.43 (r2 = 0.9999)，平均加样回收率99.4%，RSD2.12%（n=3）。检测结果表明，不同产地药材中绿原酸的含量有一定的差异，变化范围在32.84μg/g6.62μg/g。结论：该方法简便、准确、重复性好，可用于开口箭药材的质量控制。 关键词 开口箭；绿原酸；HPLC；含量测定 Determination of chlorogenic acid in Tupistra chinensis by HPLC WANG Xing ZHANG Na ZHAO Hua* （Bio-Resources Key Laboratory of Shaanxi Province Shaanxi University of Tecnology Hanzhong 723000，China）Abstract：Objective: To established a method for determination of chlorogenic acid in Tupistra chinensis. Method: The chlorogenic acid were extracted from Tupistra chinensis using 50％ methanol, and the conditions of the ultrasonic wave as follows: extraction ultrasonic power of 240W, ultrasonic frequency of 40KHz, temperature of 60℃ and ultrasonic treatment time of 30 min. Samples were analyzed on an Inertsil ODS-3C18 column（4.6×150mm，5μm)using acetonitrile-0.4%phosphoric acid as mobile phase，speed of flow was 1.0mL/min，the examination wave length was 327nm and the column temperature was 30℃. ResultChlorogenic acid showed a good linearity in the range of 0.255~3.06μg / mL，egression equation was Y=31388X+152.43(r=0.9999）and the average recovery was 99.4%（RSD2.12%）. The result showed that there are some different in the content of chlorogenic acid in Tupistra chinensis. among different origins. Conclusion: The established method is simple，credible，accurate，repeatable and can be used for the quality control of Tupistra chinensisKey words：Tupistra chinensis；chlorogenic acid；HPLC；determination of the content 开口箭属 (Tupistra)隶属于百合科铃兰族。该属植物主要分布于亚洲， 约 20种， 我 通讯作者：^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ 基金项目：^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^