黄芩苷的色谱分析.docVIP

黄芩中黄芩苷亚临界水提取及姜黄超临界提取物高效液相分析 Study on SBWE of Baicalin from Radix Scutellariae and Identification of Curcuminoids in Supercritical CO2 Extract from from Curcuma longa L. by HPLC Analysis 黄芩中黄芩苷亚临界水提取及姜黄超临界提取物高效液相分析摘要建立了黄芩药材中黄芩苷的亚临界水提取-高效液相色谱测定方法。分别考察了温度、压力、提取时间、提取物颗粒度、溶剂比等因素对提取量的影响，并与有机溶剂提取法比较。结果表明当两者具有相同的提取效果时，亚临界水提取法的提取时间及提取溶剂的消耗量大大减少，避免了使用有机溶剂造成的污染。该技术有望成为从中药材中提取脂溶性成分的有效方法。 用液相色谱-质谱和紫外-可见光谱对姜黄超临界提取物中的3种主要成分进行了定性分析，用高效液相色谱进行了定量分析。结果表明超临界CO2 萃取的姜黄提取物中含对二脱甲氧基姜黄素、脱甲氧基姜黄素和姜黄素，姜黄提取物中的总姜黄素含量约为90%。 关键词：亚临界水提取高效液相色谱黄芩姜黄高效液相色谱-质谱Study on SBWE of Baicalin from Radix Scutellariae and Identification of Curcuminoids in Supercritical CO2 Extract from from Curcuma longa L. by HPLC Analysis Abstract A method for the extraction of baicalin from Radix Scutellariae with subcritical water coupled to HPLC analysis was established. The effects of various parameters, which included the pressure, temperature, extraction time, and the particle size of the plant material as well as the solvent/ sample ratio on the yield were investigated. Compared to the conventional organic solvent extraction method, the subcritical water extraction method showed shorter handling time and lower solvent consumption without waste producing. This technique seems very promising for the extraction of liposoluble substances from plant materials. HPLC-MS, UV- VIS adsorption spectrum and HPLC- MS were employed to analyze the supercriticalCO2 extract of Curcuma longa L. Experimental results showed that curcumin, desmethoxycurcumin and bismethoxycurcumin were contained in the supercritical CO2 extract of Curcuma longa L. More than 90% of total curcuminoids was identified in the supercritical CO2 extract of Curcuma longa L. Key words：baicalin,Curcuma longa L.,HPLC- MS 前言 近年来超临界流体萃取(supercritical fluid extraction,SFE)技术又逐渐引起人们的重视，该技术大都采用超临界的CO2作为萃取剂，主要是因为CO2的低毒性及对环境无污染。但CO2 由于非极性和相对分子质量小的特点对许多强极性和相对分子质量大的物质缺乏足够的溶解性而提取效率不高[1]。其最典型的表现是，它对于近年来越来越受到人们重视、对癌症和心脑血管等疾病有显著疗效的多糖类、皂苷类、黄酮