07-第三讲蛋白质结晶-2011范例.ppt

* * * * * * * * * * * * * * * * * * * * * * * * HTP Crystallization Screening - Cartesian Honey Bee Genomic Solutions Optimization - Douglas Instruments ORYX Imaging - Discovery partners Crystal Farm Diffraction Quality - Rigaku/MSC ACTOR Sitting drop Microbatch Screening Capacity 4608 setups/day (8hr) 12 proteins per day Imaging Capacity 460,788 images/month 10 proteins per day Optimization Capacity 1200 setups/day 25 proteins per day X-ray Screening Capacity 108 crystals/day 9 - 12 proteins per day Complexes: protien-ligand, protein-protein, protein-DNA, protein-RNA etc Membrane proteins: hot pots, detergent screen, CNS papers Glycosylated protein Protein Complex Crystallization Crystallization of protein-protein complexes, S. Radaevand P. D. Sun ,J. Appl. Cryst. (2002). 35, 674-676? Crystallization of RNA-Protein Complexes By: Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, Kiyoshi Nagai /Abstract/doi/10.1007/978-1-59745-209-0_13 Crystallization of protein-DNA complexes, Wei Yang, Ph.D.,Laboratory of Molecular Biology, NIDDK, NIH, / nihxray/Tips-and-Tricks_Crystallization_Protein-DNA.html Membrane Proteins Crystallization Strategies for crystallizing membrane proteins , Journal of Bioenergetics and Biomembranes, 1996, 28(1), 13-27 Membrane Protein Purification and Crystallization, Second Edition: A Practical Guide (Spiral-bound), Academic Press,2003 Etc… ALWAYS TRIAL AND TRY Glycosylated protein An efficient platform for screening expression and crystallization of glycoproteins produced in human cells, nature protocols,/2009/04/02/an_efficient_platform_for_scre.php Protein Glycosylation, Sweet to Crystal Growth?? Jeroen R.?Mesters* and Rolf?Hilgenfeld, Cryst. Growth Des., 2007, 7 (11), pp 2251–2253 Summary Crystallization will always present a problem, no crystals – no crystal structure Take advantage what others have found useful Don’t be afraid to use your own imaginative or innovative approaches Crystallization Databases are design