AOTF近红外光谱技术在淫羊藿提取过程在线检测中的应用.docVIP

AOTF近红外光谱技术在淫羊藿提取过程在线检测中的应用 　　[摘要] 目的 研究声光可调滤光器（AOTF）近红外光谱技术，以实现淫羊藿提取过程的在线检测。 方法 采用AOTF近红外光谱仪，在淫羊藿提取的生产过程中实时采集近红外光谱，实时取样，采用紫外分光光度法进行离线检测。通过一阶微分 PLS算法建立校正模型，利用外部验证和内部验证的方法考察模型预测的准确性。 结果 分别对提取过程3次煎煮提取液总黄酮含量建立模型，其相关系数分别为0.9699、0.9811、0.9059，内部验证的平均偏差为-0.28%、-0.21%、1.43%，外部验证的平均偏差分别为-0.72%、-0.26%、3.54%。 结论 AOTF近红外光谱技术可有效地应用于淫羊藿提取过程的在线检测和质量控制。 　　[关键词] 声光可调滤光器；近红外光谱；在线检测；淫羊藿 　　[中图分类号] R284.2 [文献标识码] A [文章编号] 1674-4721（2014）08（b）-0004-04 　　[Abstract] Objective To study the near-infrared spectroscopy technology of acousto-optic tunable filter （AOTF） in order to achieve online detection for Epimedium brevicornum Maxim extracting process. Methods Near infrared spectrometer of AOTF was used to real-time collect near-infrared spectroscopy in production process of Epimedium brevicornum Maxim extraction.Offline detection was conducted by real-time sampling，ultraviolet spectrophotometry.The calibration s was created by first-order differential PLS algorithm，the accuracy of model prediction was inspected by the method of external validation and internal validation. Results The model of total flavonoids content of 3 times decoction extract of extracting process was built respectively，and correlation coefficient was respectively 0.9699，0.9811，0.9059， the average deviations of internal validation respectively was -0.28%，-0.21%，1.43%， the average deviations of external validation respectively was -0.72%，-0.26%，3.54%. Conclusion AOTF near-infrared spectroscopy technology can be effectively applied to online detection and quality control for Epimedium brevicornum Maxim extract process. 　　[Key words] Acousto-optic tunable filter；Near-infrared spectroscopy；Online detection；Epimedium brevicornum Maxim 　　淫羊藿为小檗科植物，具有很高的药用价值，味辛、甘，温，归肝、肾经，主治功能：补肾阳，强筋骨，祛风湿。其提取液中主要含有黄酮类化合物、木脂素、生物碱、挥发油等[1]。对淫羊藿提取液的评价主要以其中总黄酮的含量为标准，总黄酮的含量检测以分光光度法计算而得。如要进一步得到其中淫羊藿苷的含量则需要使用高效液相色谱法[2]，此种方法需要消耗大量药品及试剂，检测时间较长，检测结果滞后，不能满足现代化大生产在线质量控制的需求。药物制剂质量的控制不仅对最终产品进行质量控制，更要对整个工艺流程中内单元环节的产品实现动态实时在线的质量控制[3]。 　　近红外光谱技术是近年来迅