AOTF近红外光谱技术在淫羊藿浓缩过程在线检测中的应用.docVIP

AOTF近红外光谱技术在淫羊藿浓缩过程在线检测中的应用 　　[摘要] 目的 研究声光可调滤光器（AOTF）近红外光谱技术，以实现淫羊藿浓缩过程的在线检测。 方法 采用AOTF近红外光谱仪，在淫羊藿提取液的浓缩工艺实时采集近红外光谱，通过实时取样，紫外分光光度法离线检测。通过一阶微分和偏最小二乘法（PLS1）建立校正模型，利用外部验证和内部验证的方法考察模型预测的准确性。 结果 经AOTF近红外光谱技术得到的光谱数据和离线检测数据关联性很好，相关系数达到0.98；所建立模型在预测淫羊藿浓缩过程中总黄酮含量时的绝对偏差，内部验证为0.596 mg/mL，外部验证为1.380 mg/mL，均符合浓缩过程中能接受的偏差范围。 结论 AOTF近红外光谱技术在实际生产过程中可有效实现实时在线质量控制，从而解决离线分析结果滞后的缺陷。 　　[关键词] AOTF近红外光谱；在线检测；淫羊藿 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2014）08（c）-0111-04 　　Detection of concentration from Epimedium brevicornum Maxim. in processing line with AOTF near-infrared spectroscopy 　　YANG Li LI Jing LIU Cuihong XU Dingzhou LI Yu ZHONG Ming 　　Guangzhou Hanfang Pharmaceutical Co.， Ltd.， Guangdong Province， Guangzhou 510240， China 　　[Abstract] Objective To achieve online detection of the Epimedium brevicornum Maxim. concentration process using the AOTF near infrared spectroscopy. Methods The near infrared spectroscopy of Epimedium brevicornum Maxim. in the concentration process was obtained with acousto-optic tunable filter （AOTF） near-infrared spectrometer. Meanwhile the real-time sample was measured using off-line UV spectrophotometer. The calibration models were built up by adopting the first-order differential equation and partial least-squares （PLS1） and the accuracy of the models was evaluated through external validation and internal validation. Results The near-infrared spectroscopy data and the off-line UV-detected data were correlated and the result was satisfactory. The correlation coefficient was 0.98. The relative deviation of the flavonoids concentration was 0.596 mg/mL via internal validation and 1.380 mg/mL via external validation using the established model， which satisfied the demands of the concentration progress. Conclusion AOTF near-infrared spectroscopy technology provides effective real-time monitoring on the product quality， solving the problems of hysteresis with off-line detection. 　　[Key words] AOTF near-infrared spectroscopy； Online detection；