ETV1在胃肠道间质瘤中的特异性表达.docVIP

ETV1在胃肠道间质瘤中的特异性表达 　　【摘要】 目的：探讨ETV1在胃肠道间质瘤组织中的表达及临床意义。方法：应用免疫组化、实时荧光定量PCR、Western-blot检测ETV1在135例不同胃肠道间质瘤患者的肿瘤组织及瘤旁组织中的表达。结果：免疫组化结果显示，ETV1在胃肠道间质瘤中阳性表达率为68.1%，显著高于瘤旁正常组织的5.2%（P0.05）。荧光定量PCR方法显示，间质瘤组织中ETV1的相对表达Ct值（3.30±2.37）低于瘤旁正常组织的（6.19±2.86），肿瘤组织中ETV1的表达是正常组织的7.41倍，差异有统计学意义（P0.001）。Western-blot法检测显示，ETV1蛋白在胃肠道间质瘤中的表达水平（1.24±0.67）也较瘤旁正常组织高（0.88±0.28）（P=0.0369）；Pearson相关分析显示，ETV1mRNA和蛋白水平呈正相关（r=0.526，P=0.017）。结论：ETV1在胃肠道间质瘤中特异性高表达，可能与胃肠道间质瘤的发生发展有关，有望成为新的肿瘤标志物，为临床治疗提供新靶点。 　　【关键词】 胃肠道间质瘤； 转录调控因子； 肿瘤发生 　　【Abstract】 Objective：To investigate the expression of ETV1 in gastrointestinal stromal tumors and its clinical significance.Method：Surgical gastrointestinal stromal tumor tissues and adjacent normal mucosa were obtained from 135 patients with gastrointestinal stromal tumor.Immunohistochemistry was conducted to detect the distribution of ETV1 protein，while the quantitative real-time reverse transcriptase polymerase chain reaction and Western blot method were used to detect the expressions of ETV1 mRNA and protein.Result：Immunohistochemical analysis showed the positive rate of ETV1 expression was 68.1% in gastrointestinal stromal tumor tissues，it was significantly higher than 5.2% in the adjacent normal samples（P0.05）.Fluorescence quantitative polymerase chain reaction （PCR） showed that relative expression of Ct value was （3.30±2.37）in gastrointestinal stromal tumor tissues，it was significantly lower than （6.19±2.86） in adjacent normal samples，expression of ETV1 in tumor tissue was 7.41 times of normal tissue，the difference was statistically significant（P0.001）.Western blot analysis revealed that the expression of ETV1 protein was （1.24±0.67） in gastrointestinal stromal tumor tissues，it was significantly higher than （0.88±0.28） in normal colorectal tissues （P=0.0369）.Pearson correlation analysis showed that the mRNA level was in positive correlation with the protein level（r=0.526，P=0.017）.Conclusion：The specific expression of the ETV1 is in a correlation with the p