第二次实验-RT-PCR技术幻灯片.pptVIP

RT-PCR(Reverse Transcription-Polymerase Chain Reaction) PCR(Polymerase Chain Reaction) 1. What is PCR? PCR is an in vitro method of DNA synthesis by which a particular fragment of DNA can be specifically amplified. 2 Background knowledge of PCR: DNA replication DNA denaturation, renaturation hybridization DNA Replication: DNA Denaturation Renaturation: Hybridization 3 Principles of PCR 高温变性 低温退火 适温延伸 循环25-30次 4 The Characteristics of PCR High specificity High sensitivity Simple and rapid operation Wide applicability 引物设计的原则 引物长度：15-30bp 引物中碱基分布：尽可能随机分布，G+C占45%～55% 引物自身不能互补 引物之间不能互