1分子生物学常用技术3PCR幻灯片.pptVIP

* /chemistry/laureates/1993/mullis-lecture.html /chemistry/laureates/1993/mullis-autobio.html Mullis, K.B. (1990) The unusual origin of the polymerase chain reaction. Scientific American. 262 (4) 56-65. * Good laboratory technique. Note that ethanol is not an effective way to clean you work area. DNA is not soluble in ethanol. We in fact use it to precipitate DNA. 10% bleach is probably the best approach as it will hydrolize as well as dissolve the DNA. Any consumer pump spray like 409 or Fantastic are less noxious and effective alternatives. Screw cap tubes prevent spray of your samp