2.第二章基因工程的“工具”课件.pptVIP

Enzymes are the Tools of DNA Technology The tools of DNA technology are the same enzymes used by cells to modify their own DNA: DNA Polymerases DNA Ligase One special class of enzyme is pivotal to the cloning of DNA and many other techniques used in DNA Technology These enzymes are the restriction endonucleases Restriction - Because for the way they work, they restrict bacteriophages to only one host bacterial strain. They are also restricted to acting on only specific DNA sequences Endonuclease - They cut nucleic acids in the middle not just the ends Restriction Endonucleases There are a num