iData_紫杉醇组合生物合成的进展_李杰要素.pdf

生 物 工 程 学 报 李杰 等/ 紫杉醇组合生物合成的研究进展 355 Chinese Journal of Biotechnology /cjbcn March 25, 2014, 30(3): 355?367 DOI: 10.13345/j.cjb.130393 ?2014 Chin J Biotech, All rights reserved 综 述 紫杉醇组合生物合成的研究进展 李杰，王春梅 北京中医药大学生物制药系，北京 100102 李杰, 王春梅. 紫杉醇组合生物合成的研究进展. 生物工程学报, 2014, 30(3): 355–367. Li J, Wang CM. Advances of taxol combinatorial biosynthesis. Chin J Biotech, 2014, 30(3): 355–367. 摘 要: 紫杉醇是植物次级代谢产物中一种具有抗癌作用的萜类物质。但由于在自然界中含量低、难获得 使其成本昂贵，临床应用受限。近年来，利用组合生物合成技术制备紫杉醇成为研究热点，研究用的体系 以大肠杆菌、酵母为主，还涉及到小立碗藓、拟南芥、番茄、人参等植物体系。文中综述了不同体系中紫 杉醇的组合生物合成研究进展，分析了目前存在的问题，并对不同体系的发展方向提出建议。最后，结合 本实验室对紫杉醇组合生物合成的小立碗藓体系的研究，阐述了其发展前景和优势。 : 紫杉醇，组合生物合成，大肠杆菌，酿酒酵母，小立碗藓，拟南芥，番茄，人参 Advances of taxol combinatorial biosynthesis Jie Li, and Chunmei Wang Department of Biological Pharmaceutics , Beijing University of Chinese Medicine, Beijing 100102, China Abstract: Taxol is a kind of isoprenoid with strong anticancer activity. It is difficult to be obtained because of its low concentration in nature, which hinders its application in cancer treatment. Recently, biosynthesis methods for taxol production have attracted more attentions. Several systems including Escherichia coli , Saccharomyces cerevisiae, Physcomitrella patens , Arabidopsis , tomato and ginseng were explored. This review focuses on the advance in biosynthesis of taxol in different systems and features the bottleneck of scale fermentation for producing the intermediates. At the same time some advices for the further were given. At last, the future and character of Physcomitrella patens system used in taxol combinatorial biosynthesis were analyzed based on our lab’s research. Keywords: taxol, combin