泰利霉素对巨噬细胞亚群调控妊娠期弓形虫感染所致的不良妊娠结局的影响机制(终).docVIP

泰利霉素对巨噬细胞亚群调控妊娠期弓形虫感染所致的不良妊娠结局的影响机制 摘要：目的：探究泰利霉素对巨噬细胞亚群调控妊娠期弓形虫感染所致的不良妊娠结局的影响机制。方法：筛选2014年1月~2015年8月入我院行弓形虫抗体检测的孕妇，将弓形虫抗体检查阳性孕妇随机分为A组（n=39）和B组（n=42），并从检测结果阴性的孕妇中随机抽取50例为C组（即n=50）。观察对比三组腹腔巨噬细胞培养上清液中TNF-α、IFN-γ，IL-4的浓度和巨噬细胞M1/M2亚型比值，记录自然流产、死胎死产、出生缺陷等不良妊娠结局数量及发生率，记录A组服药过程中不良反应情况。结果：与C组相比，A、B两组TNF-α、IFN-γ，IL-4水平均明显升高（P0.05），但与B组相比A组TNF-α、IFN-γ，IL-4水平均明显较低（P0.05）。与C组相比A、B两组M1/M2比值明显较大（P0.05）而与A组与B组相比M1/M2比值明显较小（P0.05）。与C组相比，A组、B组合计不良妊娠结局发生率均明显较高（P0.05），B组的自然流产、死胎死产、出生缺陷发生率均较C组高（P0.05）。与B组相比A组各不良妊娠结果发生率均明显较小（P0.05）。结论：泰利霉素能通过影响巨噬细胞分化方向有效降低妊娠期弓形虫感染所致的不良妊娠结局。 关键字：泰利霉素；巨噬细胞；分化；弓形虫；不良妊娠 The mechanism of Telithromycin on adverse pregnancy outcomes of T.gondii infection by macrophage regulation Abstract: Objective: To explore the mechanism of telithromycin influence the adverse pregnancy outcome caused by T. gondii infection in the pregnancy period , which is regulated by macrophage subsets.Methods: pregnant women from Jan 2014 to Aug 2015 for T.gondii antibodies testing were selected in our hospital.T.antibody positive pregnant women were randomly divided into group A (n= 39) and group B (n = 42), and 50 women whose result is negative were randomly selected called group C (n = 50). Result：Compared with the group C , TNF-α, IFN-γ, IL-4 levels in groups A and groups B were significantly elevated (P0.05), but compared with the group A levels of TNF-α,IFN-γ, IL-4 in group B were significantly lower (P0.05).Compared with the C group, the M1/M2 ratio of A and B was significantly larger (P0.05) and the M1/M2 ratio was significantly smaller (P0.05) than that in B group compared with group A（P0.05）.Compared with group C, the adverse pregnancy outcome incidence of group A and B were significantly higher (P 0.05). The rate of spontaneous abortion, stillbirth, born defect of group B were higher than those in group C (P 0.05). The incidence of adverse pregnancy outcomes in B group was significantly smaller than that in A group (P0.05).Conclusion: Telithromycin can effectively reduce