Molecular Biomimetics Synthesizing Gold 分子仿生合成金.pptVIP

Molecular Biomimetics:Synthesizing Gold Nanostructures with Amino AcidsAlexander ChenChemical and Environmental Engineering Department, UC Riverside Reasons For Using Biomimetics Simple and efficient method that creates consistent nanostructures and patterns in easily controllable environments. Environmentally Safe- no harmful waste are produced in comparison to chemical methods of synthesis. Has potential for greater compatibility and efficiency for integration within biological environments during medical use. Process of Synthesizing Gold Nanostructures with Amino Acids The nanostructures are synthesized through mixing AuCl4 and specific amino acids within an aqueous solution and incubated (Mimicking physiological environments in which typical synthesis reactions occur). The gold structures that are formed are based on the specific binding properties of each particular amino acid and the control of concentration, contents of solution, pH, and temperature. Synthesis Conditions and Procedure: 1mL samples consisting of 940?L water, 40?L 0.5mM or 20mM AuCl4, and 10?L amino acid (Alanine, Arginine, Asparagine, Aspartic acid, Cysteine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Proline, Serine,Threonine, Tryptophan, Tyrosine, and Valine) and 1 control sample with no amino acid Excess chloride concentration were set at 0N, 0.1N, and 0.5N for various sets. 25M NaOH used to adjust pH to 3 and 5 for 0.5mM AuCl4 and to 3.2, 4.5, 5.2, 7.5, and 11.6 for 20mM AuCl4 - Initial pH of each individual sample is recorded to ensure uniformity between samples (±0.1) - Solutions are incubated for 3 days at 37°C - Centrifuged and washed for three cycles at 10 rpm and 10min/cycle. - Final pH after the reaction are measured to examine correlation with synthesizing and binding efficiency. 10ppm of amino acid solution mixed with HCl(10%) to create 5mL solutionand measured gold ion concentration in