Sordaria Linkage Mohawk Valley Community College粪壳联动莫霍克谷社区学院.pptVIP

Sordaria Linkage The Inheritance of Spore Color Sordaria Life Cycle I. Spore color A. Natural spore color is black B. Produced by the action of two genes C. The alleles are represented as: 1. t+ for the wild type 2. t for the mutant (which by itself produces tan 3. g+ for the wild type 4. g for the mutant (which by itself produces gray. 5. if both mutants are present-the phenotype of the ascus is clear D. Possible genotypes and resultant phenotypes 1. recall that fungi normally exist in the haploid state 2. therefore no such thing as dominance and recessiveness 3. g+t+ produces black 4. gt+ produces a gray spores 5. g+t produces tan spores 6. gt produces clear spores 7. you do not know if the genes collaborating for spore color are on the same or different chromosomes II. Tan X Black A determination of cross over frequency A. tg+ X t+g+ tan X black B. Since the second allele is the same in both we can ignore the “g” allele for this exercise C. We will be trying to measure the percent crossing over between the “t” gene and its centromere D. Normally 1. notice parental types are haploid 2. opposite mating hypha would undergo plasmogamy to produce the dikaryotic stage 3. karyogamy follows within the asci followed by meiosis I and II 4. in this example crossing over did not occur 5. no crossing over is indicated by four spores of one type followed by four spores of the second E. If crossing over happens, spore arrangement in the asci will be different than 4:4 1. Events are the same initially as the slide before 2. Now crossing over occurs at prophase I 3. Sister chromatids of the two chromosomes are now modified 4. Will result in a different spore pattern laid down in the asci Are any other spore patterns possible that would indicate that crossing over has occurred? a. How would you get a “2:4:2” pattern? To calculate the percent crossing over a. make a smash of the sordaria b. count parental asci c. recombinant asci d. % crossove