Surface Plasmon Resonance and SPR Imaging UITS表面等离子体共振和SPR成像单元.pptVIP

Surface Plasmon Resonance Imaging for Biosensor Applications Presented by Michelle Brideau March 31, 2005 CHEM 395 Outline Surface Plasmon Resonance SPR background SPR imaging SPR imaging experiments Conclusions Advantages of SPR Future applications Surface Plasmon Resonance (SPR) Label-free method Surface sensitive spectroscopic technique Used to detect the binding of biological molecules onto arrays of probe biomolecules covalently attached to chemically-modified gold surfaces Typical Signal from SPR Measurement How does SPR work?  This Kretschmann Experimental System uses a metal film thin enough to monitor the plasmon Otto Experimental System (1968) Metals Gold and silver are most commonly used. Aluminum and copper may also be suitable metals. SPR A surface-plasmon-resonance is excited at a metal-dielectric interface by a monochromatic, p-polarized light beam, such as He-Ne laser beam The surface plasmon is sensitive to changes in the environment near the interface and therefore has potential as a sensing probe. Sensitive detection method that monitors variations in thickness and refractive index in ultra-thin films Refractive Index Ratio of velocity of propagation of electromagnetic wave in vacuum to velocity in medium More about SPR It is a non-destructive means of sensing Surface Plasmons have already been used for gas sensing, biosensing, immuno-sensing and electrochemical studies. Modifications for SPR imaging apparatus The p-polarized He-Ne laser beam passes through a spatial filter and can be expanded using a beam expander The reflected light is collected by a CCD camera to produce an “SPR image” SPR imaging apparatus Spatially-filtered, expanded, p-polarized HeNe laser beam illuminates the gold sample through a prism coupler. Reflected light from the gold surface, containing the SPR image, is monitored with a CCD camera. The angle of incidence can be changed by rotating the entire sample assembly. SPR Imaging Experiment Investi