中华肿瘤投稿版肺炎衣原体感染在H.docVIP

肺炎衣原体感染在HEp-2细胞黏附和迁移中的作用＊ 张利军 洪莉 陈宁 沈炳玲 邓艳秋 权伟 王蓓蓓 张丽莙 【摘要】 目的 观察肺炎衣原体（C.pn）感染在人喉癌细胞系（HEp-2）细胞黏附和迁移中的作用，以期阐明C.pn感染在肿瘤转移中的作用及机制。方法 C.pn增殖培养后感染HEp-2细胞, 光镜下观察细胞病变，吖啶橙染色观察细胞内C.pn包涵体的形态特点，透射电镜观察C.pn包涵体超微结构。细胞黏附实验观察C.pn感染对HEp-2细胞黏附能力的影响。创伤修复实验和Transwell实验检测C.pn感染HEp-2细胞后，细胞迁移能力的改变。结果 C.pn感染HEp-2细胞72 h后，细胞内的空泡状结构即包涵体几乎占据整个胞浆。吖啶橙染色结果显示，细胞内的包涵体呈黄绿色葡萄串状。透射电镜观察到包涵体内成熟的梨形原体（EB）明显增多，而圆形的网状体（RB）则较少。细胞黏附实验结果显示，C.pn感染HEp-2细胞2 h后，C.pn感染组黏附细胞的吸光度（A）值0.669±0.011，明显高于对照组的0.558±0.005（P0.001），C.pn感染组的细胞黏附率为119.89%。创伤修复实验结果显示，C.pn感染HEp-2细胞24 h后，细胞向划痕中央迁移的距离明显长于对照组（P0.05）。Transwell实验结果显示，C.pn感染HEp-2细胞12 h后，C.pn感染组的细胞迁移数目明显多于对照组（P0.001）。结论 C.pn感染能增强HEp-2细胞的黏附能力，同时还能促进HEp-2细胞迁移，提示C.pn感染可能在喉癌转移过程中起促进作用。 【主题词】 喉肿瘤；肺炎衣原体；细胞黏附；细胞迁移；肿瘤转移 Role of Chlamydia pneumoniae infection in HEp-2 cell adhesion and migration ZHANG Li-jun, HONG Li, CHEN Ning, SHEN Bing-ling, DENG Yan-qiu, QUAN Wei, WANG Bei-bei, ZHANG Li-jun. Department of Pathophysiology, Basic Medical College, Tianjin Medical University, Tianjin 300070, China Corresponding author：Zhang Li-jun, E-mail: lijunwz@ 【Abstract】 Objective To observe the effect of Chlamydia pneumoniae (C.pn) infection on human epidermoid carcinoma-2（HEp-2) cell adhesion and migration, to further clarify the role and mechanism of C.pn infection in tumor metastasis. Methods HEp-2 cells were infected with C.pn after the culture and propagation of C.pn. Cytopathic effect was observed with a microscope. Morphological characteristics of C.pn inclusions in HEp-2 cells were examined under fluorescence microscope by acridine orange staining. The ultrastructural changes of C.pn inclusions in HEp-2 cells were investigated by transmission electron microscope (TEM). Cell adhesion assay was performed to investigate the effect of C.pn infection on the adhesion of HEp-2 cells to collagenⅠ. The wound-healing assay and Transwell assay was performed to explore the effect of C.pn infection on HEp-2 cell migration. Results At 72 h post-infection, C.pn infected-HEp-2 cells were swollen