The Characteristics of Model Organisms模型生物特征.pptVIP

The Characteristics of Model Organisms Lab Manual—2b Plan for Lab Tues. (1 hr early)—Duffy will start yeast broth Tuesday—Finish Lecture, Parts I, II III Wednesday—FIRE, Part IV Thursday—repeat Part IV/start write-up Model Organisms Relatively easy to grow and maintain in a restricted space Relatively easy to provide necessary nutrients for growth Relatively short generation time (birth?reproduction?birth) Relatively well understood growth and development Closely resemble others organisms or systems Examples Animal Kingdom—rats, mice (lots of offspring is a +) Bacteria—Escherichia coli (E. coli)-prokaryotes Fungal—Aspergillus (mold)--eukaryotes Yeast—Saccharomyces cerevisiae Lab Write-up Purpose Materials Safety Precautions Procedures Data Collection Analysis (table 2.3—add 8C, only 2 trials to average) Conclusion (aka Data Analysis) REE-results, evidence, explanation PE—possible error PA—practical applications Thinking Like a Biotechnician Background of Purpose To grow and study an organism in a laboratory, one should know the environmental preferences of the species. Maintaining an organism at less than optimal light intensity, temperature, or oxygen level, from example, may put the organism under stress, possibly affecting its growth or other processes. Purpose What are the temperature preferences shown by three model organisms (E. coli, Aspergillus niger and baker’s yeast) grown in a biotechnology lab? Prep. For Lab 2B Month Prior Check supplies: bleach, petri dishes, LB base, inoculating loops, 3 incubators, yeast, glucose, foil Order: E. coli, Aspergillus, Potato plates Week Prior Perez will prepare LB agar broth Pour plate (2 per group?20) Set incubators at 30, 37 and 42 C Scalpels from other room 1 day prior make bacteria culture Pre-lab—Plate Pouring Pour LB agar base plates (Luria-Bertani ) E. Coli plate—use the LB agar with sterile technique, use a 10ml pipette to transfer 10ml into a sterile plate Yeast plate—use the LB* agar, sterile techniqu